Description
The blood sample contains a large number of red blood cells and platelet cells, which makes blood processing more troublesome, and the purity and quantity of blood genomic DNA purified by conventional methods is low. The kit uses Foregene Protease Plus and unique BL1 and BL2 buffer systems, so anticoagulated blood and coagulated blood samples can be completely digested in a short time, thereby minimizing DNA degradation and obtaining the maximum amount of genomic DNA. The fast blood processing system and simple spin column operation greatly simplify the purification of blood genome, and high-quality and high-purity genomic DNA can be obtained within 40 minutes.
The DNA-only silica gel membrane used in the spin column of the kit is Foregene's unique new material, which can efficiently and specifically bind DNA, and can remove RNA, impurity proteins, ions and other organic compounds in cells to the greatest extent. The maximum capacity of the spin column is 80 μg DNA. The obtained DNA can be used in molecular biology experiments, such as restriction digestion, PCR, Southern hybridization and DNA library construction.
Specification
|
Kit contents
|
DE-05111
|
DE-05112
|
DE-05113
|
|
50 T
|
100 T
|
250 T
|
|
Buffer BL1
|
15 mL
|
30 ml
|
75 ml
|
|
Buffer BL2 *
|
15 mL
|
30 ml
|
75 ml
|
|
Buffer DC
|
100 mL
|
200 ml
|
500 ml
|
|
Buffer PW *
|
25 mL
|
50 ml
|
125 ml
|
|
Buffer WB1
|
15 mL
|
30 ml
|
75 ml
|
|
Buffer EB
|
10 mL
|
20 ml
|
50 ml
|
|
Foregene Protease Plus
|
1.25 mL
|
2.5 ml
|
6.5 ml
|
|
DNA-Only Column
|
50
|
100
|
250
|
|
Manual
|
1
|
1
|
1
|
*: Buffer BL2 and Buffer PW contain irritating liquid salt. Please wear gloves and take relevant protective measures when operating.
Features&advantages
-No RNase contamination: The DNA-Only Column provided in the kit makes it possible to remove RNA from genomic DNA without additional RNase during the experiment, thereby preventing the laboratory from being contaminated by exogenous RNase.
-Fast speed: Foregene Protease has higher activity than similar proteases and digests tissue samples faster;
-Simple: the genomic DNA purification operation can be completed in 50 minutes.
-Convenient: The centrifugation is performed at room temperature, 4℃ centrifugation and ethanol precipitation of DNA is not required.
-Safety: no organic reagent is used.
-High quality: The purified genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.
Kit application
It is suitable for extraction and purification of genomic DNA from fresh or frozen anticoagulated whole blood.
Storage and Shelf life
The kit can be stored for 12 months at room temperature (15-25℃), or 2-8℃ for longer time.
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