AMSBIO: Safeguarding Organoids Through Lockdown 

Hits:568   Date: 11/12/2020

AMSBIO reports how the internationally renowned Hans Clevers Lab at the Hubrecht Institute, Netherlands used their CELLBANKER® reagent when they needed to freeze down their organoids at short notice ahead of the COVID-19 spring lockdown.

As COVID-19 cases continue to rise, public health orders and advisories have forced many scientists to freeze down their experiments, to keep them safe while they are kept away from their laboratories.

AMSBIO’s CELLBANKER® and STEM-CELLBANKER® freezing solutions provide a simple and economical solution in such emergencies, as well as for routine archiving – they can even be used to cryopreserve cultured cells, organoids or other 3D structures in situ in the original culture plate.

Preserving organoids through Lockdown using CELLBANKER®

Jelte van der Vaart, a Ph.D researcher in the Hans Clevers Lab commented about the ease of freezing their valuable organoids with CELLBANKER®. He said "Historically our lab had always used the FBS/DMSO based freezing method to preserve our organoids. However, this methodology not only is very time consuming but also requires transfer by slow cooling to -80°C to liquid nitrogen. On the day of the lockdown, me and some other researchers froze our organoids down for the first time in CELLBANKER®. The procedure was a lot easier and faster since I could simply transfer the organoids in CELLBANKER® to the freezer immediately. This beneficially also solved the limited availability of Mr Frosty’s which was a problem because we all had to freeze down lines at the same time. When we were able to return to our lab after the lockdown, the organoids looked similar after thawing to standard freezing methods and could be easily retrieved from personal drawers instead of shared Mr Frosty drawers. CELLBANKER® genuinely improved the process of freezing and safely preserving our organoids when going into lockdown".
 

Human Airway Organoids frozen in CELLBANKER 1® during lockdown and imaged post-thaw. IF for DAPI, KRT5 (basal cell marker), Phalloidin (blue/red/green). Lumens highlighted in yellow in right-hand image. Images courtesy of the Clevers Lab, Hubrecht Institute.
 

To read the full story of how CELLBANKER® made it easy to cryopreserve precious organoids when the Hans Clevers Lab went into lockdown and how the organoids still expressed appropriate markers and were capable of further expansion when defrosted please visit https://www.amsbio.com/news/cellbanker-organoid-cryopreservation/. For further information about CELLBANKER® please visit https://www.amsbio.com/cellbanker-cell-freezing-media/ or contact AMSBIO on +44-1235-828200 / +1-617-945-5033 / info@amsbio.com.  

The Hans Clevers Lab is internationally renowned for its pioneering research studies into the molecular mechanisms of tissue development and cancer of various organs using organoids made from adult Lgr5 stem cells. For further information please visit https://www.hubrecht.eu/research-groups/clevers-group/ 

Founded in 1987, AMS Biotechnology (AMSBIO) is recognized today as a leading transatlantic company contributing to the acceleration of discovery through the provision of cutting-edge life science technology, products and services for research and development in the medical, nutrition, cosmetics and energy industries. AMSBIO has in-depth expertise in extracellular matrices to provide elegant solutions for studying cell motility, migration, invasion and proliferation. This expertise in cell culture and the ECM allows AMSBIO to partner with clients in tailoring cell systems to enhance organoid and spheroid screening outcomes using a variety of 3D culture systems, including organ-on-a-chip microfluidics. For drug discovery research, AMSBIO offers assays, recombinant proteins and cell lines. Drawing upon a huge and comprehensive biorepository, AMSBIO is widely recognised as a leading provider of high-quality tissue specimens (including custom procurement) from both human and animal tissues. The company provides unique clinical grade products for stem cell and cell therapy applications these include high quality solutions for viral delivery (lentivirus, adenovirus and adeno-associated virus) in addition to GMP cryopreservation technology.

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