Ecalbio Launches Tetrodotoxin ELISA kit and rapid test kit

Ecalbio research  Tetrodotoxin ELISA kit and rapid test kit, which can widely used to detect tetrodotoxin (TTX) residues in porcupinefish, ocean sunfish or mola origin food samples, incorporates a fast, low-cost sample prep protocol with high recovery rates compared with LC-MSMS ot HPLC method.

 

The direct competitive enzyme-linked immunosorbent assay (dcELISA) kit was developed for detecting tetrodotoxin (TTX). The working conditions of the dcELISA kit including the anti-TTX mAb coating concentration, coating method, enzyme-labeled antigen concentration, the antigen diluents, reaction time and temperature were all optimized. The result showed that mAb coating concentration was 3.72 μg/ml, it was coated at the condition of minimal power treatment of microwave oven for 3 min. The enzyme-labeled antigen concentration was 4.08 μg/ml. The competitive reaction was under the condition of room temperature 25 °C for 30 min. The half maximal inhibitory concentration (IC50) of the standard curve was 20.4 ng/ml, detection limit was 1.1 ng/ml, linear range 3.3～137 ng/ml, the intra-assay CV and inter-assay CV were 6.25% and 7.34% respectively. And recovery rate of TTX ranged from 65.0% to 93.2% with the CV of 9.41～12.77%. This method is convenient, sensitive and time-saving, hope this dcELISA kit can bring benefits and reference for TTX detection.

 

At the same time, Ecalbio scientist also manufacture Tetrodotoxin (TTX) Rapid Test Kit, is a sandwich lateral flow immunochromatographic assay  for the qualitative detection of Tetrodotoxin (TTX) in the sample within 10 min, the lowest detect limit can be up to 0.5 MU (0.10 µg )/mL for sample extraction solution, 10 MU (2.0 µg )/mL for sample.

 

Tetrodotoxin ELISA kit , 96 tests /kit

Tetrodotoxin rapid test, 40 tests/Kit