Azenta Life Sciences: Two-component Plate Design Improves qPCR Results

Azenta Life Sciences reports how the two-component design of its FrameStar® microplate range reduces thermal expansion and sample evaporation leading to improved consistency of PCR results.

FrameStar 96 Well Skirted PCR Plate
 

Many commercially available PCR and qPCR plates are based on polypropylene designs because of its chemical inertness and that ultra-thin tube walls can be moulded enabling fast temperature transfer. While polypropylene is the standard material for PCR consumables, thermal expansion and evaporation properties reduce its suitability for applications including qPCR and Next Generation Sequencing. FrameStar PCR plates employ a novel two-component design consisting of polypropylene tubes in a polycarbonate frame.

In a pivotal study – the authors demonstrate how FrameStar PCR plates minimize the problem of evaporation related qPCR inaccuracies due to their two-component design. The reduced risk of evaporation using these two-component plates facilitates the miniaturisation of reaction volumes and therefore provides opportunities for savings relating to lower use of expensive PCR reagents.

Comparing risk of evaporation in wells during a (q)PCR run in a one-component PP plate(left) and a two-component PP (wells) and PC (frame) plate (right). Red denotes high risk, yellow is medium risk and green (low risk).

Study data also demonstrated the benefits of using polycarbonate in the FrameStar PCR plate design. Using a more rigid plate frame which does not alter size or shape during PCR reduces distortion and weakens seal attachment to produce more reliable data for quantification.
FrameStar PCR plates are available in 96- and 384-well formats, with a choice of multiple well-frame colour combinations. The 384-well plates are recommended for low volume PCR. The 96-well plates are available in low and standard (high profile) and come with a variety of skirt lengths (non, semi, and fully skirted).

To download a copy of the study please visit https://web.azenta.com/hubfs/azenta-files/resources/posters-publications/41004-PP-Influence-PCR-Consumables.pdf. For further information on FrameStar PCR plates please visit https://www.azenta.com/4titude-framestar-pcr-plates

Azenta, Inc. is a leading provider of life sciences solutions worldwide, enabling impactful breakthroughs and therapies to market faster. Azenta provides a full suite of reliable cold-chain sample management solutions and multiomics services across areas such as drug development, clinical research and advanced cell therapies for the industry's top pharmaceutical, biotech, academic and healthcare institutions globally. Our global team delivers and supports these products and services through our industry-leading brands, including GENEWIZ, FluidX, Ziath, 4titude, Limfinity, Freezer Pro, Barkey, and B Medical Systems. Azenta is headquartered in Burlington, MA, with operations in North America, Europe, and Asia. For more information, please visit www.azenta.com.

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Company
 
AZENTA Life Sciences 
Consumables & Instruments Group
22 Gilchrist Road
Northbank Industrial Estate
Irlam
Manchester M44 5AY
UK
Tel: +44 -161-777-2098 
Web: http://www.azenta.com