Understanding and Using Isothermal Gene Assembly for Synthetic Biology

Latest issue of IDT’s Decoded newsletter explains the fundamentals of artificial gene construction

 

CORALVILLE, IA (5^th March 2012) – Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, provides a unique insight into the fundamentals of isothermal synthetic gene assembly in this quarter’s edition of the company’s Decoded newsletter. The technique, which was pioneered in 2009 by Daniel Gibson and colleagues at The J. Craig Venter Institute in Maryland, USA, allows several overlapping DNA fragments to be combined in a single reaction. In this way, large synthetic DNA molecules many hundreds of kilobases long can be generated using a minimal number of enzymatic steps. This limits potential errors due to repeated handling of samples and reagents, while simplifying the process by avoiding the need for multiple experimental setups. The article in the latest issue of Decoded outlines the theory behind isothermal assembly, and provides advice for designing and creating synthetic gene constructs using the method.

 

You can read more about the latest genomic trends, tips and research in the most recent issue of IDT’s quarterly newsletter, Decoded. IDT has also released its new gBlock Gene Fragments, which simplify, speed up and reduce the cost of creating large, custom DNA constructs.

 

For more information about IDT’s complete product portfolio, and to sign up to receive Decoded, visit www.idtdna.com. For real-time news and updates, follow us on Twitter @idtdna.

 

About IDT

Integrated DNA Technologies (IDT) is the largest supplier of custom nucleic acids in the United States, serving academic, government, and commercial researchers in biotechnology, clinical diagnostics, and pharmaceutical development. IDT's primary business is the manufacture of custom, synthetic DNA and RNA oligonucleotides. Today, IDT synthesizes and ships an average of 36,000 custom oligos per day to more than 86,000 customers worldwide. IDT manufacturing locations include facilities in Coralville, Iowa; San Diego, Calif.; and Leuven, Belgium. For more information visit www.idtdna.com.

 

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