The 3rd Advanced Training Workshop in the Technology and Applications of Proteomics

Beijing Proteome Research Center

The 3^rd Advanced Training Workshop in the Technology and Applications of Proteomics

 

Proteomics is revealing the secret of life and has found wide applications in all disciplines of life science. It is becoming the frontier and breakthrough point in life science and biotechnology in the 21^st century. To further facilitate the development of proteomics in China, Beijing Proteome Research Center (BPRC), State key Laboratory of Proteomics (SKLP) and China Human Proteome Organization (CNHUPO) will hold jointly the 3rd Advanced Training Workshop in the Technology and Applications of Proteomics from July 19 to 22, 2011 in Zhongguancun Life Science Park.

 

BPRC was founded in 2004. It hosts the main body of the SKLP and is the executive headquarter of the International Human Liver Proteome project (HLPP). BPRC is the leader of Chinese proteomics research and has established an internationally renowned research platform for modern proteomics. Many national projects of the 973 and 863 Plans, the Chinese human liver proteome project, and projects funded by the National Natural Science Foundation (in total of more than 100 projects) have been and are being carried out in BPRC.

 

This training workshop will be taught by experts from BPRC who have been working on the forefront of proteomics research. Lectures on “Technology and Methodology for Proteomics, Technology know-how and Experimental Design, Frontiers and Hot Topics in Proteomics Research” will be given on July 19 and 20. On July 21 and 22, trainees will watch live demonstrations of key proteomics techniques. A certificate of completion of the training in proteomics will be issued for all participants.

 

1.Training Syllabus

Lectures

Theory, Strategy and Application of Proteomics   Dr Xiaohong Qian

Sample Preparation in Proteomics    Dr Ying Jiang

Separation Technology in Proteomics   Dr Yangjun Zhang

Biological Mass Apectrometry and Application in Proteomics    Yun Cai

Bioinformatics in Proteomics    Dr Yunping Zhu

Methods and Applications of Quantitative Proteomics    Dr Wantao Ying

Experimental Design in Quantitative Proteomics    Dr Ping Xu

Techniques and Applications of Protein-Protein Interactions   Dr Jian Wang

Data Analysis of Protein-Protein Interactions    Dr Dong Lee

Technology and Application of Pepdomics   Dr Kaihua Wei

Frontiers and Hot Topics in Proteomics    Dr Jun Qin

 

Tour of BPRC

Experimental Demonstration (half a day per item)

 

1) 2DE-DIGE

Different protein samples are labeled with fluorescent dyes of two different colors so that they can run together in gel electrophoresis. This reduces the experimental error, increases sensitivity and the dynamic range of the detection. The software package can automatically detect proteins of differential expression. This method is suited for differential proteomics of multiple samples with large differences in protein expression among individual samples.

 

2) Techniques in Chromatography

Analytical high efficiency liquid chromatography, capillary high efficiency liquid chromatography, and their in-line use with mass spectrometry will be discussed. Emphases will be given on the components of liquid chromatography, the basic functions of the various components, basic operation and handling of the liquid chromatography, potential problems and trouble shooting in the operation of liquid chromatography. After the lecture, you should have a basic understanding of liquid chromatography and its use in separation of peptides and proteins.

 

3) Operation of a MALDI TOF/TOF Mass Spectromter

We will teach you how to: desalt the sample, prepare the matrix solution, apply samples, set parameter and check hardware, generate and edit the sample table, control the laser, manually acquire data, automatically acquire data in a batch mode, calibrate the instrument, smoothen and correct background, label peaks, use the DataExplore software, operate the mass spectrometer, export data, maintain database, and perform routine instrument maintenance.

 

After the session, you should have a basic idea of how to use MALDI-TOF/TOF mass spectrometry.

 

4) Protein identification with an in-line liquid chromatography electro-spray mass spectrometry (LC-ESI-MS)

 

The technique of LC-ESI-MS/MS is the method of choice for the identification of proteins. It is well suited for complex mixture of peptides. The working horse in the field is capillary reverse phase liquid chromatography, coupled with a Nano-spray source, in-line with a liner Ion Trap mass spectrometer (LTQ). Such combination has proven to be efficient and accurate, and it can be used to identify proteins continuously with high sensitivity. We will cover the keys to optimized protein identification techniques in this session: the condition for best HPLC separation and parameter settings for mass spectrometry, as well as parameter settings for data base search.

 

Logistic Details

 

1) A certificate issued by BPRC and SKLP will be presented at the end of the workshop.

 

2) BPRC is certified by ISO/IEC 17025(CNAS-CL01), and provides scientific and technological services in research and development for academics and industries alike. As a participant of this training workshop, you are eligible to obtain these services with favorable pricing. Please visit our website:

www.bprc.ac.cn/guidance/list.php?catid=27  for details.

 

3) As a participant of this training workshop, you are also eligible to purchase a product from Beijing C&N International Sci-tech Co., Ltd. at a discount. Please visit the websites for details:

www.bprc.ac.cn/guidance/show.php?itemid=21    

www.bprc.ac.cn/guidance/show.php?itemid=29

 

Time and Place

 

On-site registration:

July 18, 13:30 to 21:00, Beijing Yangtze River Pharmaceutical Group, Hainuokang Guild Hall（No.16, Lifee Park Road, Changping District, Beijing,China）

 

July 19, 8:30-10:00, BPRC (No.33, Science Park Road, Changping District, Beijing,China）

 

Training Session:

July 19 to 22, BPRC.

 

Registration and Fees

 

Online Registration: http://61.50.138.116/training/cn/

Advanced Registration: June 17, 2011

 

The registration includes meeting fee, training material, and food. Food and accommodation are arranged by the organizer. Accommodation is an extra. Please fill in the information online if you need accommodation arrangement.

 

Workshop Tuition:

（1）Lecture

￥1300 (￥1100 for students), if you register before June 17, 2011.

￥1400 (￥1200 for students), if you register between June 18 and July 19, 2011.

（2）Experimental Demonstration

￥650/item/half day (￥450 for students) if you register before June 17, 2011.

￥700/item/half day (￥500 for students) if you register between June 18 and July 19, 2011.

Student ID is required for student discount.

 

Payment

Account Number：0200004909200041055  

Name of Account：Beijing Proteome Research Center (BPRC)

Bank: Industrial and Commerce Bank of China, Beijing Yongding Road Branch

Note: please write clearly your name and place of work when wiring the payment. Pay to “Training Workshop of Proteomics”. Please fax your receipt of fund wiring to BPRC, or e-mail a scanned PDF to sky_proteomics@sina.com.

 

Contacts

 

Beijing Proteome Research Center

State key Laboratory of Proteomics

China Human Proteome Organization

 

Contact Person and Phone:

Registration:Ms. Jianping Zhou  86-10-80727777-1512   

Information:Ms. Dongmei Shi    86-10-80727777-1520, 86-10-80705888

Fax:86-10-80705155

E-mail: sky_proteomics@sina.com  

Mailing Address:No.33, Science Park Road, Changping District, Beijing, 102206, China