Multiplex Fluorescence IHC Staining Kits for Tumor Microenvironment Research

1. Background Introduction
Immunohistochemical staining is a common technique for studying tissue morphology and in situ protein expression. With the rise of immunotherapy, an increasing number of markers in the tumor microenvironment have been found to be closely related to disease treatment and progression. Simultaneous staining and labeling of these markers, as well as research on their colocalization, expression levels, and distance relationships, have become universal needs in tumor immunology research.

The multiplex staining kits from the Absin product line of ANT BIO PTE. LTD. optimize the combination of antibodies and fluorescent dyes using Tyramide Signal Amplification (TSA) technology, developing a series of fluorescent immunohistochemical kits that can simultaneously label and detect multiple biomarkers.

2. Product Overview
The tumor microenvironment consists of tumor cells, cancer-associated fibroblasts, immune cells, secretory products of the above cells (such as cytokines and chemokines), and non-cellular components in the extracellular matrix. The Tumor Microenvironment Series Staining Kits can label keratin (pan-CK), T cells, myeloid-derived immune cells, and immune checkpoint proteins. Each kit allows the selection of any 3 markers from the above.

3. Application
It is mainly used for immunofluorescent staining of Tumor-Infiltrating Lymphocytes (TILs) in the tumor microenvironment research series.

4. Technical Principle
Tyramide Signal Amplification (TSA) technology uses HRP-labeled secondary antibodies. HRP catalyzes the fluorescent substrate added to the system to generate activated fluorescent substrates. The activated substrates can covalently bind to tyrosine on the antigen, enabling stable covalent binding of fluorescein on the sample. Subsequently, heat-induced epitope retrieval is used to wash away non-covalently bound antibodies, followed by incubation with the next primary antibody in the second round and replacement with another fluorescent substrate. This cycle is repeated to achieve multiplex labeling.

5. Experimental Flow Chart
Sample Preparation → Incubation with Primary Antibody 1 → TSA Reaction (Dye 1) → Antibody Elution → Incubation with Primary Antibody 2 → TSA Reaction (Dye 2) → Antibody Elution → Incubation with Primary Antibody 3 → TSA Reaction (Dye 3) → Antibody Elution → DAPI Staining → Imaging

6. Product Advantages
High Scientific Research Value: Available in both human and mouse series, facilitating parallel experiments on mouse tumor models and human samples, and supporting translational medicine research.
High Compatibility: The selected fluorescent dye labels are suitable for all fluorescence microscopes, confocal microscopes, and fluorescence section scanning equipment equipped with ultraviolet, FITC, CY3, CY5, and other wavelength bands.
Stability: No light protection is required during the sample addition process. After staining, the samples can be stored at 4°C for half a year and at -20°C for one year, and the fluorescent signals still meet the photography requirements.
Convenience: All antibodies have been screened for good specificity, and the staining concentrations have been optimized, reducing the process of researchers optimizing conditions by themselves.

7. Product Recommendation

Catalog No.	Product Name	Specification
abs50086	Two-Color Multiplex Immunofluorescence IHC Staining Kit (Anti-Rabbit Secondary Antibody)	100T
abs50087	Two-Color Multiplex Immunofluorescence IHC Staining Kit (Mouse/Rabbit Universal Secondary Antibody)	100T
abs50088	Three-Color Multiplex Immunofluorescence IHC Staining Kit (Anti-Rabbit Secondary Antibody)	100T
abs50089	Three-Color Multiplex Immunofluorescence IHC Staining Kit (Mouse/Rabbit Universal Secondary Antibody)	100T
abs50012	Four-Color Multiplex Immunofluorescence IHC Staining Kit (Mouse/Rabbit Universal Secondary Antibody)	20T/50T/100T
abs50168	Four-Color Multiplex Immunofluorescence IHC Staining Kit B (Anti-Rabbit Secondary Antibody)	20T/50T/100T
abs50013	Five-Color Multiplex Immunofluorescence IHC Staining Kit (Mouse/Rabbit Universal Secondary Antibody)	20T/50T/100T
abs50029	Five-Color Multiplex Immunofluorescence IHC Staining Kit (Anti-Rabbit Secondary Antibody)	20T/50T/100T
abs50030	Six-Color Multiplex Immunofluorescence IHC Staining Kit (Anti-Rabbit Secondary Antibody)	20T/50T/100T
abs50048	Six-Color Multiplex Immunofluorescence IHC Staining Kit (Plus) (Anti-Rabbit Secondary Antibody)	20T/50T/100T
abs50049	Six-Color Multiplex Immunofluorescence IHC Staining Kit (Plus) (Mouse/Rabbit Universal Secondary Antibody)	20T/50T/100T
abs50015	Seven-Color Multiplex Immunofluorescence IHC Staining Kit (Mouse/Rabbit Universal Secondary Antibody)	20T/50T/100T
abs50031	Seven-Color Multiplex Immunofluorescence IHC Staining Kit (Anti-Rabbit Secondary Antibody)	20T/50T/100T
abs50037	Seven-Color Multiplex Immunofluorescence IHC Staining Kit (Plus) (Mouse/Rabbit Universal Secondary Antibody)	20T/50T/100T
abs50038	Seven-Color Multiplex Immunofluorescence IHC Staining Kit (Plus) (Anti-Rabbit Secondary Antibody)	20T/50T/100T
abs50165	Seven-Color Multiplex Immunofluorescence IHC Staining Kit (770 Dye Enhanced Version) (Anti-Rabbit Secondary Antibody)	20T/50T/100T
abs50166	Seven-Color Multiplex Immunofluorescence IHC Staining Kit (770 Dye Enhanced Version) (Mouse/Rabbit Universal Secondary Antibody)	20T/50T/100T
abs50018	Ten-Color Multiplex Immunofluorescence IHC Staining Kit	100T
abs50083	Lung Cancer Tumor Microenvironment Multiplex Immunofluorescence IHC Detection Kit (I)	20T
abs50084	Lung Cancer Tumor Microenvironment Multiplex Immunofluorescence IHC Detection Kit (II)	20T

8. Brand Mission
ANT BIO PTE. LTD. is dedicated to empowering life science research through the provision of high-quality, reliable reagents and comprehensive solutions. Our specialized sub-brands (Absin, Starter, UA) are strategically positioned to cover a full spectrum of research needs: Absin focuses on general reagents and kits, Starter specializes in antibodies, and UA is dedicated to recombinant proteins. We strive to support researchers in unlocking scientific mysteries, advancing medical progress, and addressing unmet clinical needs through continuous innovation and customer-centric services.

9. Disclaimer
This article is AI-compiled and interpreted based on the original work in document 1226.docx (Multiplex Fluorescence IHC Staining Kits). All product-related data and technical information are for research purposes only and are not intended for clinical diagnosis or treatment. For any product quality issues, please contact us promptly and we will handle them actively.

10. Brand Promotion Copy
ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs
At ANTBIO, we are committed to advancing life science research through high-quality, reliable reagents and comprehensive solutions. Our specialized sub-brands (Absin, Starter, UA) cover a full spectrum of research needs, from general reagents and kits to antibodies and recombinant proteins. With a focus on innovation, quality, and customer-centricity, we strive to be your trusted partner in unlocking scientific mysteries and driving medical progress. Explore our product portfolio today and elevate your research to new heights.