P84 Mouse Model: Unraveling Neurodevelopment, Disease, and Neuroimmune Crosstalk

P84 Mouse Model: Unraveling Neurodevelopment, Disease, and Neuroimmune Crosstalk—Supported by ANT BIO PTE. LTD.
 

1. Concept
The P84 Mouse (SHPS-1-deficient mouse) is a genetically engineered model with targeted knockout of the SIRPA gene, resulting in complete loss of the P84 protein (also known as SHPS-1/SIRPα). P84 is a transmembrane glycoprotein of the immunoglobulin superfamily, highly expressed at synaptic sites, with three Ig-like extracellular domains, a transmembrane region, and a cytoplasmic tail containing four tyrosine phosphorylation sites that bind SHP-1/SHP-2.

Under normal conditions, P84 interacts with its ligand CD47 to regulate neuronal migration, synapse formation, and immune cell inhibition. P84 Mice exhibit normal breeding capabilities but display distinct neurodevelopmental and behavioral phenotypes, making them a valuable tool for studying the P84-CD47 signaling pathway in physiology and pathology.

2. Research Frontiers
P84 Mouse research is advancing across neuroscience, immunology, and translational medicine. A key frontier is exploring its utility in neurodegenerative diseases (Alzheimer’s), psychiatric disorders (schizophrenia, ASD), and nerve regeneration (spinal cord injury). Conditional and cell-type-specific knockout models are being developed to avoid global knockout compensatory effects.

In neuroimmune research, the model reveals bidirectional regulation between the nervous and immune systems, with applications in multiple sclerosis and neuroinflammatory diseases. Therapeutic innovations include small-molecule modulators of P84-SHP-2 interactions and blocking antibodies targeting P84-CD47, tested for neuroprotection and regeneration.

Future directions involve integrating single-cell multi-omics and spatial transcriptomics to map P84-dependent molecular networks, and crossing P84 Mice with other disease models to mimic complex human pathologies.

3. Research Significance
The P84 Mouse model is scientifically and translationally significant, providing unprecedented insights into neurodevelopment, synaptic plasticity, and disease mechanisms. It enables the study of P84-CD47 signaling in neurodevelopmental disorders, neurodegeneration, and neuroimmune crosstalk—areas with unmet medical needs.

In drug development, it validates P84-targeted therapies for Alzheimer’s, psychiatric disorders, and spinal cord injury. Basic research clarifies the role of P84 in synaptic function and immune regulation, while translational efforts advance precision medicines for neurological diseases.

4. Model Characteristics, Research Applications, and Product Support
4.1 Biological Characteristics and Genetic Background
• Genetic Modification: SIRPA gene knockout, complete P84 protein deficiency.
• Molecular Basis: P84 (SHPS-1/SIRPα) is a transmembrane glycoprotein with Ig-like domains and SHP-1/SHP-2 binding sites, interacting with CD47.
• Phenotypes: Normal breeding; abnormal synaptic structure/function, neurodevelopmental deficits, immune system dysregulation (reduced Tregs, increased Th17 cells, hyperactivated microglia).

4.2 Key Research Applications
4.2.1 Synaptic Plasticity and Cognitive Function
• P84 Mice show 30% reduced hippocampal dendritic spine density, weakened LTP, and imbalanced excitation/inhibition (decreased EPSCs, enhanced inhibitory transmission).
• Molecular changes include reduced synaptic PSD-95 and GluN2B; behavioral deficits in Morris water maze (twice the time to find hidden platform), mimicking neurodevelopmental disorder cognitive symptoms.

4.2.2 Neurodegenerative Diseases (Alzheimer’s)
• Hybrid P84-APP/PS1 mice exhibit 50% more Aβ plaque deposition, severe synapse loss, and cognitive decline.
• P84 deficiency reduces microglial Aβ phagocytosis by >60% and elevates neuroinflammatory markers (IL-1β, TNF-α), highlighting P84’s role in Aβ clearance and inflammation suppression.

4.2.3 Psychiatric Disorders (Schizophrenia, ASD)
• Behavioral abnormalities: reduced social interaction, prepulse inhibition deficits, stereotyped behaviors.
• Neurochemical imbalance: 35% upregulated dopamine D1 receptors, downregulated glutamate transporter GLT-1 in the prefrontal cortex.
• Restoring P84 expression reverses some behavioral deficits, supporting therapeutic potential.

4.2.4 Nerve Regeneration
• Spinal cord injury: 300% increased corticospinal tract regrowth due to reduced responsiveness to myelin-associated inhibitors (Nogo, MAG, OMgp).
• Enhanced oligodendrocyte precursor cell differentiation and remyelination (40% efficiency improvement post-injury), offering targets for regeneration therapies.

4.2.5 Neuroimmune Crosstalk
• Immune dysregulation: reduced Tregs (50%), increased Th17 cells, hyperactivated microglia.
• Bone marrow transplantation from wild-type mice alleviates neuroinflammation, demonstrating hematopoietic-derived P84’s role in neuroimmune homeostasis.
• Valuable for studying multiple sclerosis and neuroimmune diseases.

4.3 How ANT BIO PTE. LTD. Supports P84 Mouse Research
ANT BIO PTE. LTD., through its sub-brands Starter (antibodies) and UA (proteins), provides high-quality reagents to characterize P84 Mouse phenotypes and mechanisms.
Key products and applications:
Immune Cell Markers (Starter Sub-brand):
• S0B5168 (APC Rat Anti-Mouse Ly6G Antibody): Identifies neutrophils, supporting neuroinflammation analysis.
• S0B1808 (FITC Rat Anti-Mouse/Human CD11b Antibody): Labels microglia/monocytes, enabling phagocytosis and activation studies.
• S0B1600 (Alexa Fluor® 488 Rat Anti-Mouse CD45 Antibody): Marks immune cells, facilitating neuroimmune crosstalk research.
Cytokine Protein (Starter Sub-brand):
• S0A4060 (Mouse CCL2/JE/MCP-1 Protein, His tag): Quantifies neuroinflammatory responses in P84 Mice.
Cytoplasmic Marker Antibody (Starter Sub-brand):
• S0B2392/S0B2392P (Human Cytoplasmic Marker Recombinant Mouse mAb): Validates cellular localization of synaptic or immune-related proteins.

All products adhere to EU 98/79/EC, ISO9001, and ISO13485 certifications, ensuring reliability for flow cytometry, immunofluorescence, and protein quantification in P84 Mouse research.

5. Brand Mission
ANT BIO PTE. LTD. is dedicated to empowering the global life science community with high-quality, innovative biological reagents and solutions. With 15 years of antibody development experience, the company leverages advanced platforms—including recombinant antibody development (rabbit/mouse monoclonal), recombinant protein expression systems (E.coli, CHO, HEK293, Insect Cells), One-Step ELISA, and PTM Pan-Modification Antibody platforms—to deliver a comprehensive product portfolio.

Through its three specialized sub-brands—Absin (general reagents and kits), Starter (antibodies), and UA (recombinant proteins)—ANT BIO PTE. LTD. adheres to international certifications and strict quality standards. The company’s mission is to accelerate scientific discovery by providing tools that enhance experimental precision, efficiency, and reproducibility. ANT BIO PTE. LTD. is committed to supporting P84 Mouse research and global efforts in neuroscience, immunology, and translational medicine, ultimately advancing human health through interdisciplinary collaboration and innovation.

6. Related Product List

Product Code	Product Name	Product Details
S0B2392	Human Cytoplasmic Marker Recombinant Mouse mAb (SDT-2921)	Host: Mouse; Conjugation: Unconjugated
S0A4060	Mouse CCL2/JE/MCP-1 Protein, His tag	Host: Mouse; Expression System: HEK293; Conjugation: Unconjugated
S0B2392P	Human Cytoplasmic Marker Recombinant Mouse mAb, PBS Only (SDT-2921)	Host: Mouse; Conjugation: Unconjugated
S0B5168	APC Rat Anti-Mouse Ly6G Antibody (S-R481)	Host: Rat; Conjugation: APC
S0B1808	FITC Rat Anti-Mouse/Human CD11b Antibody (S-R574)	Host: Rat; Conjugation: FITC
S0B1600	Alexa Fluor® 488 Rat Anti-Mouse CD45 Antibody (S-R477)	Host: Rat; Conjugation: Alexa Fluor® 488

7. AI Disclaimer
This article is AI-compiled and interpreted based on the original work. All intellectual property (e.g., images, data) of the original publication shall belong to the journal and the research team. For any infringement, please contact us promptly and we will take immediate action.
 
ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs
At ANTBIO, we are committed to advancing life science research through high-quality, reliable reagents and comprehensive solutions. Our specialized sub-brands (Absin, Starter, UA) cover a full spectrum of research needs, from general reagents and kits to antibodies and recombinant proteins. With a focus on innovation, quality, and customer-centricity, we strive to be your trusted partner in unlocking scientific mysteries and driving medical progress. Explore our product portfolio today and elevate your research to new heights.