Product Description

Chromatrap® enzymatic shearing kit supplies all the necessary reagents and buffers for up to 10 chromatin preparations. The success of a ChIP assay is highly dependent on the quality of chromatin prepared.  Chromatrap® enzymatic shearing kit  allows you to determine optimal shearing conditions for your chromatin preparations and can supply you with enough chromatin to perform up to 24 ChIPs if using standard Chromatrap®  ChIP spin column kit or up to 96 IP’s if using Chromatrap®  ChIP 96 High throughput microplate.

Kit Contents

1.3M Glycine  20mls
Hypotonic Buffer 10mls
Digestion Buffer 10mls
Enzymatic Stop Solution 200µl
Lysis Buffer 10mls
5M NaCl 500µl
1M NaHCO3 500µl
Shearing Cocktail 100µl
Protease Inhibitor Cocktail (PIC) 100µl
Proteinase K  50µl
Proteinase K Stop Solution 100µl

Porvair Sciences, specialists in the manufacture of microplate products, serve Life Sciences, Biotechnology, R&D and Molecular Biology with microplate solutions for all applications, from sample preparation to high throughput screening via our global distributor network.

Our range includes vacuum manifolds, sealers, evaporators and microtiter plates in all popular styles; deep well and shallow well storage plates, assay plates, luciferase reporter gene plates and liquid handling reagent reservoirs. We also provide custom microplate products for life science research.

Our vacuum manifolds, essential for 96-well SPE sample preparation, are designed to work with most popular filter plates, including Waters, Millipore, Qiagen, Whatman, GE Healthcare, Varian, Biotage and, of course, Porvair.

For further information, including custom built microplates, reagent reservoirs, vacuum manifold design or microplate handling equipment, please contact us: Tel: +44 1978 666240; Email: int.sales@porvair-sciences.com.