Analytical column (Affinity, DNA, Sugar, SEC， Empty tube),specification,price,image-Bio-Equip in China

Product Detail
Company Profile

Prosep protein A affinity chromatographic column
The costones protein a analytical column is designed for rapid detection of monoclonal antibody (mAb) concentration (titer)
Design. The product uses the principle of affinity chromatography and the specific binding ability of alkali resistant recombinant protein a ligand and immunoglobulin Fc fragment to achieve accurate capture and quantification of antibodies in complex fermentation broth. Different from the traditional agarose matrix, prosep series uses highly crosslinked hydrophilic polystyrene divinylbenzene (psdvb) particles as the matrix:

High mechanical strength: high pressure resistance is significantly improved, supporting higher flow rate without deformation.
 good mass transfer efficiency: it has higher dynamic binding flux (DBC) and significantly reduces the analysis cycle time.
 long service life: stronger physical stability and chemical resistance, significantly reducing the cost of a single test.
Core advantages
 seamless connection of HPLC system: standard chromatographic column specifications are adopted, which can be directly used on conventional hplc/uhplc instruments.
 rapid analysis cycle: a single analysis takes only 2 – 5 minutes, which greatly improves the high-throughput screening efficiency of upstream process development.
 excellent linear range: maintain excellent linear relationship in the wide concentration range of 0.02 – 10 mg/ml, and reduce sample dilution operation.
 very low non-specific adsorption: ensure high accuracy of quantitative results and effectively deal with complex cell culture medium components.
 excellent alkali resistance: it can withstand 0.1 – 0.5 M NaOH strong alkali cleaning, effectively prevent cross contamination and prolong the service life of the column.

project	Prosep protein A affinity chromatographic column	Prosep protein a plus affinity chromatographic column
Chromatographic column tube	2.1mm ID × 30mm L; 4.6mm ID × 50mm L	2.1mm ID × 30mm L; 4.6mm ID × 50mm L
Column pipe material	316L stainless steel column, PEEK	316L stainless steel column, PEEK
stroma	Hydrophilic polystyrene divinylbenzene (PS-DVB)	Hydrophilic polystyrene divinylbenzene (PS-DVB)
Ligand	Modified protein A	Modified protein A
Average particle size	40μm	20μm
Storage solvent	0.02M disodium hydrogen phosphate, pH 7.0， 0.02% sodium nitrite	0.02M disodium hydrogen phosphate, pH 7.0， 0.02% sodium nitrite
PH range	pH 2-10	pH 2-10
Withstand voltage	1000 psi	1000 psi
Cleaning solvent	0.1-0.5M NaOH	0.1-0.5M NaOH
Cycle time	2-5 minutes	2-5 minutes
Service temperature	4-40 ℃	4-40 ℃

Product item No

Product name	specifications	Stainless steel column	PEEK column
Prosep A	2.1*30mm	11073002103S	11073002103P
Prosep A	4.6*50mm	110730-04605S	11073004605P
Prosep A PLUS	2.1*30mm	11072002103S	11072002103P
Prosep A PLUS	4.6*50mm	11072004605S	11072004605P

DNA Analysis Column
Based on the most advanced column technology, the DNA column is designed for the efficient separation and accurate characterization of oligonucleotides (including dna/rna, mRNA and plasmid) by liquid chromatography and LC-MS.
DNA RP column: highly crosslinked macroporous ps/dvb microspheres, suitable for the separation of macromolecular DNA and RNA.
 DNA 200-c18: monodisperse C18 coupled silica gel for the separation of small oligonucleotides.
 DNA 1000-c18: monodisperse C18 coupling silica gel is used for the separation of macromolecular oligonucleotides, DNA and RNA.
Core advantages
 monodisperse microspheres with high column efficiency, good pressure resistance and good separation selectivity
 good chemical stability, low column loss, good consistency between columns

project	DNA RP	DNA 120-C18	DNA 1000-C18
Ligand	Quaternary ammonium group	Octadecyl	Octadecyl
stroma	PS-DVB	Spherical silica gel	Spherical silica gel
Average particle size	5um	3um/5um	3um/5um
Average aperture	-	200A	1000A
Maximum withstand voltage	5000 psi	5um: 5000psi; 3um: 6000psi	5um: 5000psi; 3um: 6000psi
Upper temperature limit	80℃	50℃	50℃
PH range	2-12	2-11	2-11

Specifications	4.6×250mm	4.6×150mm	2.1×150mm	2.1×50mm
DNA RP	D30105000004625	D30105000004615	-	-
DNA 120-C18	D00303002004625	D00303002004615	D00303002002115	D00303002002105
DNA 1000-C18	D00305010004625	D00305010004615	D00305010002115	D00305010002105

Sugar analytical column
Sugar analytical chromatographic column is specially designed for efficient analysis of various polysaccharides, sugar alcohols and organic acids. This series of chromatographic columns uses high-performance monodisperse polystyrene divinylbenzene (psdvb) microspheres as the matrix, and combines the advanced coordination exchange and ion exchange principles to provide excellent analytical power for the qualitative and quantitative analysis of complex samples.

Core Technology
Monodisperse psdvb matrix: two monodisperse polymeric microspheres with different crosslinking degrees are used, taking into account the excellent physical strength and optimized pore structure, ensuring a very long service life under high temperature and high pressure.
Unique sulfonation bonding process: through precise chemical modification, the functional groups are firmly bonded to the polymer surface to ensure a high degree of reproducibility of the analysis results.
Coordination exchange separation principle: separation is realized by using the difference of coordination between metal counter ions and sugar hydroxyl, and isomers with similar structures can also obtain ideal resolution.
H+type: analysis of mixtures of organic acids, sugar alcohols and various sugars. It is the first choice for fermentation analysis and food quality monitoring.
Na+type: separation of oligosaccharides and neutral sugars. Provide optimized elution sequence and separation efficiency for high molecular weight carbohydrates.
Ca2+type: analysis of sugar alcohols, monosaccharides (such as glucose and fructose) and components in honey and beverages. It has strong retention ability for sugar alcohols and higher resolution.
Product advantages
 full spectrum application: it covers the full range analysis from monosaccharides to complex polysaccharides.
 environment friendly: it mainly uses pure water as the mobile phase, without a large number of organic solvents, reducing operating costs and environmental protection.
 excellent stability: the polymer matrix has excellent chemical stability and can withstand a wide pH range.
Accuracy and consistency: strict production control ensures the consistency between columns and between batches, and simplifies the method validation process.

Product specification:

Product specifications	Sugar-10H	Sugar-10Ca	Sugar-10Na
functional group	-SO₃H	-SO₃Ca	-SO₃Na
stroma	Monodisperse spherical PSDVB particles	Monodisperse spherical PSDVB particles	Monodisperse spherical PSDVB particles
Average particle size	6um/8um	6um/8um	6um/8um
Crosslinking degree	0.1	0.1	0.1
PH range	1-3	5-9	5-9
Temperature resistance	< 95℃	< 95℃	< 95℃
Withstand voltage	1200psi	1200psi	1200psi
Product application	Mixtures of organic acids and alcohols	Honey and oligosaccharides	Sugars and maltose

Average particle size	Column specification	Sugar-10H	Sugar-10Na	Sugar-10Ca
6um	4.6*250mm	0170601004625	0580601004625	0190601004625
6um	7.8*250mm	0170601007825	0580601007825	0190601007825
8um	4.6*250mm	0170801004625	0580801004625	0190801004625
8um	7.8*250mm	0170801007825	0580801007825	0190801007825

SEC column
Costones SEC column is a high-performance volume exclusion chromatographic column specially designed for the separation of biological macromolecules. This series of products are separated based on the difference of molecular size, with a wide range of
It is widely used in biopharmaceutical quality control, drug development and basic research of life sciences.
Technical quality
 monodisperse high-strength silica gel: monodisperse microspheres with special design are used to provide high column efficiency and mechanical stability.
 neutral hydrophilic coating: a highly stable neutral hydrophilic film is constructed on the surface of silica gel, effectively shielding the active sites on the substrate surface.
 very low nonspecific adsorption: this coating minimizes the secondary interaction between the stationary phase and the biological analyte, ensuring excellent peak symmetry and polarity
High sample recovery.
Core advantages
 high column efficiency and high resolution: precisely controlled pore size distribution ensures high clarity in the separation of monomers, dimers and polymers.
Excellent peak shape and recovery rate: almost no residual adsorption, even complex biological samples can obtain accurate quantitative results.
 strong physical stability: high strength silica gel matrix can withstand higher operating pressure and significantly prolong the service life of chromatographic column.
 wide application: covering a variety of separation requirements from small molecule drugs and peptides to large proteins, nucleic acids and polysaccharides.
Product application
 sec-120: small molecule drugs, peptides, polysaccharide fragments, short chain oligonucleotides.
 sec-150: peptides, medium molecular weight proteins, glycans, oligonucleotides.
 sec-300: standard selection for the determination of monoclonal antibodies (mAbs) and their variants and aggregates.
 sec-500: large protein complexes, high molecular weight polysaccharides, and macromolecular nucleic acids.

Product specifications

Project	SEC-150	SEC-300	SEC-500
functional group	Diol	Diol	Diol
stroma	Monodisperse high purity silica matrix	Monodisperse high purity silica matrix	Monodisperse high purity silica matrix
Average particle size	5um	5um	5um
Average aperture	150A	300A	500A
PH range	2-8	2-8	2-8
Temperature resistance	<40℃	<40℃	<40℃
Withstand voltage	6000psi	6000psi	6000psi
Ligand range (PEG)	200-15,000	1,000-100,000	5,000-200,000
Ligand range (dextran)	1,000-50,000	5,000-150,000	20,000-500,000
Ligand range (globulin)	5,000-150,000	10,000-1,000,000	20,000-2,000,000

Product item No

Product Model	5um 7.8×300mm	5um 4.6×300mm	5um 4.6×50mm	5um 4.6×10mm
SEC-150	2130501507830	2130501504630	2130501504605	2130501504601
SEC-300	2130503007830	2130503004630	2130503004605	2130503004601
SEC-500	2130505007830	2130505004630	2130505004605	2130505004601

COSTONES provides a variety of liquid chromatographic columns according to different application environments
Specifications. Product specifications
 inner diameter 2.1mm, 3.0mm, 4.0mm, 4.6mm, 7.8mm, 10mm, 20mm, 21.2mm, 30mm, 50mm
 length: 20mm, 30mm, 50mm, 100mm, 150mm, 250mm, 300mm, 500mm (customizable)
 column tube material: stainless steel 316 L

COSTONES ，According to different application scenarios, different sieve plate styles are provided for analytical column tubes and semi prepared column tubes.

Protective column for liquid chromatography
The protective column is selected according to the specification of the liquid chromatographic column. The optional sizes are 4.6-10mm, 10-10mm and 20-10mm. The appearance of the 4.6-10mm size can be customized.

Welcome to inquire, E-mail：pangjun@costones.com