SILWET L-7600 is a polyalkyleneoxide-modified polydimethylsiloxane silicone surfactant that combines the strong hydrophobicity of organosilicon with the hydrophilicity of polyethers. It achieves ultra-low surface tension (as low as 20–25 mN/m) in both aqueous and organic solvent systems. Its unique molecular structure provides exceptional wetting capabilities (even on superhydrophobic surfaces like PTFE), along with anticoagulant, anti-hemolytic, and anti-static properties, making it a key functional additive in the development of solid-phase whole blood diagnostic reagents.Mechanism of Action

•Superior Wetting Ability: The siloxane segments rapidly adsorb onto hydrophobic interfaces (e.g., PTFE, nitrocellulose membranes), reducing the contact angle and promoting uniform liquid spreading.

 

•Anti-Red Blood Cell Interference: Inhibits nonspecific binding between red blood cells in high hematocrit (HCT) samples and solid-phase carriers, eliminating detection bias (e.g., false positives in coagulation tests).

 

•Non-Hemolytic Property: Acts gently on red blood cell membranes, preventing hemolysis and the release of hemoglobin, which can interfere with optical detection (e.g., colorimetric or fluorescent methods).

 

•Enhanced Penetration and Leveling: Accelerates reagent diffusion in dense matrices (e.g., blood clots, viscous samples), improving reaction homogeneity.

 

•Anti-Static Property: Reduces aggregation of particles (e.g., latex microspheres) caused by electrostatic adsorption, ensuring signal stability.

 

Application Scenarios

•Direct Whole Blood Detection Reagents: Added to solid-phase reaction plates or test strips (0.05%-0.2%) to block red blood cell adhesion and improve detection accuracy (e.g., CRP, D-dimer tests).

 

•Microfluidic Chips: Pre-coated onto hydrophobic microchannels (e.g., PDMS, PTFE) to optimize whole blood/plasma separation efficiency and reduce cell clogging.

 

•Lateral Flow Immunoassay Strips: Incorporated into sample pad treatment solutions (0.1%-0.3%) to accelerate whole blood penetration and inhibit red blood cell retention on the conjugate pad.

 

•Cell Lysis Enhancer: Combined with detergents (e.g., Triton X-100) at 0.05%-0.1% to enhance gentle lysis of blood/tissue cells.

 

•Optical Detection Anti-Interference: Used in colorimetric/fluorescence detection systems (0.05%-0.15%) to reduce light signal scattering caused by blood cell fragments.

S16 Surfactant_Surfactants_IVD materials_AccuSift

 

Shenzhen AccuSift Biotechnology Co., Ltd. (referred to as AccuSift) was established in January 2014. It is a technology-oriented enterprise specializing in the preparation of antigens and antibodies for IVD raw materials and food safety raw materials, as well as the development and application of biotechnology. The company has an advanced and complete production platform for single and polyclonal antibodies and has prepared a variety of small molecule hapten related to IVD raw materials, pesticides and veterinary drugs, antibiotics, mycotoxins, hormones, heavy metals, pigments, etc., and high-quality monoclonal antibodies. The company provides comprehensive antibody services, antigen design, synthesis, antibody preparation and labeling, and immunological experiments for domestic and foreign users. The company's antibody research and development department has high-quality talents, strong research and development capabilities and a quality assurance system, providing high-precision and high-stability product solutions for clinical diagnosis and food safety detection.

AccuSift adheres to the business philosophy of "integrity, innovation and progress", with the mission of "focusing on the source of precision and safeguarding the beauty of health", providing strong support for researchers in the fields of clinical diagnosis and food safety.