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(3) Prepare 2% agarose gel. Take 10 μL of the second PCR product for DNA electrophoresis. 3. Results The internal control of the PCR reaction is a single DNA band of 515 bp. Common mycoplasma contamination will produce a single DNA band of 236 bp-365 bp. Acholeplasma laidlawii contamination will produce double DNA bands of 426 bp and 219 bp. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Description | The MYCO-T™ Nested PCR Mycoplasma Detection Kit is used for qualitative detection of mycoplasma contamination in cell culture. Over 95% of mycoplasma contamination in cell culture is caused by Mycoplasma fermentans, M. orale, M. pirum, M. hyorhinis, M. hominis, M. salivariu, M. arginine, and Acholeplasma laidlawii. This kit uses nested PCR to amplify conserved gene fragments from these eight and several other common mycoplasmas in cell culture media, thereby determining the presence of mycoplasma contamination. This kit is characterized by rapid detection, high sensitivity, and high specificity. Each reaction can detect as few as 10 mycoplasma genes, with significantly improved sensitivity for Acholeplasma laidlawii, and exhibits no cross-reactivity with bacteria or cultured cells. Product composition: MYCO-TTM Nested PCR Mycoplasma Detection Kit contains 2 tubes of ready-to-use primers and 1 tube of reaction internal control. The specifications are as follows:
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| General Notes | 1. Hot-start Taq enzyme series is recommended for PCR reagents. The 2X PCR premix should be free of DNA gel electrophoresis stain. 2. PCR reactions should be performed in a clean bench to avoid cross-contamination and aerosol contamination. Nuclease-free pipette tips and reaction tubes should be used, and pipette tips with filters are recommended. 3. Hot-start Taq enzyme series is recommended for PCR reagents. The 2X PCR premix should be free of DNA gel electrophoresis stain. 4. PCR reactions should be performed in a clean bench to avoid cross-contamination and aerosol contamination. Nuclease-free pipette tips and reaction tubes should be used, and pipette tips with filters are recommended. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Storage Temp. | Store at -20℃ away from light, valid for 18 months. |
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