Product Specification| Usage | 1. For TSA dyes, please use signal amplification reaction solution diluted at 1:100 to prepare the dye working solution (currently used and prepared); DAPI prepared the working solution by diluting it with sterile water at a ratio of 1:100
2 Use of secondary antibody: The reagent kit is anti rabbit secondary antibody, please verify whether the species of the primary antibody match before the experiment. Pika universal secondary antibody, please refer to (# abs50049) | | Description | There are complex cell composition in tissue microenvironment. The phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. With the help of antibody staining, it can be presented in situ. Immunohistochemical staining is a common technology to study tissue morphology and in situ protein expression. Conventional IHC detection can only show a single index, which is difficult to show the cell composition, state and relationship in the complex tissue microenvironment, and this information is essential for the diagnosis and treatment of disease
tyramine signal amplification technology principle: similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP labeled secondary antibody. HRP catalyzes the addition of the fluorescein substrate of the system to produce activated fluorescent substrate. The activated substrate can covalently bind with tyrosine on the antigen to make the stable covalently bound fluorescein on the sample. After that, the non covalently bound antibody is washed away by the thermal repair method, and the second round of incubation is carried out after changing a primary antibody, and then changing another fluorescein substrate. In this way, multiple labeling can be realized | | General Notes | 1. This reagent kit is only used for immunohistochemistry and is not intended for any other purpose
2 This reagent kit is only for professional use
3 Appropriate protective measures should be taken to avoid contact between reagents and skin and eyes
4 Reagents that exceed their expiration date may have reduced activity, therefore they should not be used
5 If the staining components of this reagent kit are mixed with products from other companies, abnormal situations may occur during the staining process
6 Incomplete dewaxing can easily affect the dyeing effect
7 In order to prevent possible false negative and false positive results, both positive and negative controls should be conducted simultaneously during the experiment
8 All waste generated from the use of this reagent kit should be disposed of in accordance with the Medical Waste Management Regulations. | | Storage Temp. | Fluorescent dyes need to be protected from light, Store at 2-8 ℃, 1 year from receipt | | Applications | Used for immunohistochemical staining of tissues, paraffin sections, and TMA chips.Can also be used for frozen sections and cell slides with abs994 Antibody eluent (mIHC only) |
bio-equip.cn AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.
|
中文
