Detection Principle:

This kit uses double antibody sandwich ELISA technology. The specific anti human sCD14 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The sCD14 existing in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of sCD14 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, sCD14 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 2.58pg/ml

Detection Range: 125-8000 pg/ml

Recovery Range: 96-110%

Storage Method: 2-8 ℃

Standard Curve:

Background:

CD14 is mainly produced by macrophages and neutrophils, but also by dendritic cells. It is a component of the innate immune system as a recognition of bacterial lipopolysaccharide (LPS)Of the coreceptor. CD14 can bind LPS only in the presence of lipopolysaccharide binding protein. There are two forms of CD14. One is mCD14, which is anchored to the cell membrane by glycosylphosphatidylinositol, and the other is sCD14, which is a soluble form. SCD14 appears after the shedding of mCD14 (48kDa), or is directly produced by intracellular vesicle secretion (56kda). SCD14 is secreted by the liver and monocytes. At low concentrations, it is sufficient for cells that do not express CD14 to respond to LPS. MCD14 and sCD14 are also present in intestinal epithelial cells. SCD14 is also present in human milk and can regulate the growth of intestinal microbes in infants.