OMNISEC
The world’s most advanced multi-detector GPC/SEC system
Malvern Panalytical's OMNISEC is a Gel Permeation Chromatography (GPC) / Size Exclusion Chromatography (SEC) system for the measurement of absolute molecular weight, molecular size, intrinsic viscosity, branching and other parameters. It includes
OMNISEC RESOLVE, the integrated GPC/SEC module,
OMNISEC REVEAL, the integrated multi-detector module and
OMNISEC software for the characterization of synthetic and natural polymers, and proteins.
Features and benefits
OMNISEC is a powerful tool for taking control of the performance, quality and value of your product, whether that’s a synthetic polymer in industry, a natural polymer or polysaccharide used in food or pharmaceutical products, or a protein or antibody used in biopharmaceuticals.
As a research tool, it accelerates research into these molecules by providing absolute information for a wide range of applications making it an ideal tool for universities. It reduces service expense and downtime and increases productivity with easy-to-use intuitive software.
OMNISEC REVEAL
- The ultimate in advanced detection for GPC/SEC and UPLC/UHPLC
- Fully integrated temperature controlled detector module for improved detector stability and sample characterization
- More reliable measurements with market leading light scattering sensitivity
- Measure low concentration samples with a highly sensitive and stable refractive index detector
- Improved structural comparisons enabled with the unique mechanical auto balance of the differential viscometer
- See the full range of your samples UV absorbance with the UV-PDA
Overview
OMNISEC REVEAL can be used to relate a polymer’s molecular properties to its bulk characteristics in a wide range of applications, from the packaging and paint industries to the manufacturing of food additives and pharmaceuticals. Its protein characterization capabilities can be used to study the effects of oligomerization, aggregation and conjugation on activity and efficacy in academic research and biopharmaceutical development.
The ultimate in advanced detection for GPC/SEC
- Complete flexibility in detector configuration. Only purchase the detectors which add value to you now, with the security that any detector you require in the future can be installed onsite
Multi-detection SEC offers unparalleled information on the characterization of polymers, polysaccharides and proteins
- Measuring absolute molecular weight, intrinsic viscosity, hydrodynamic radius, Mark-Houwink parameters, radius of gyration, composition, conformation and more
- Rely on OMNISEC to help you understand, control and optimize your product performance
- Detection and quantification of protein aggregation to drive faster sample characterization and development
Fully integrated temperature-controlled detector module for improved detector stability and sample characterization
- Improve separations and result accuracy with the column oven and temperature-controlled integrated detector compartment – all installed as standard!
- Minimized inter-detector tubing, maintained at the same temperature as the detector, reduces band broadening effects to further increase signal quality
More reliable measurements with market leading light scattering sensitivity
Measure low concentration samples with a highly sensitive and stable refractive index detector
- Measure molecular weight of polymers as low as 200 g/mol!
- Injection masses as low as 100 ng for characterization of samples which were previously unmeasurable using advanced detection
- Make sure you don’t miss low molecular weight and low concentration contaminants or protein fragments
Improved structural comparisons enabled with the unique mechanical auto balance of the differential viscometer
- Boost lab productivity and reduce service costs with a novel self-balancing viscometer design with user exchangeable capillaries
See the full range of your samples UV absorbance with the UV-PDA
- Distinguish chemical changes by measuring absorbance across the full UV/Vis spectrum (190-900nm) using the photodiode array
- All UV channels are recorded giving you the flexibility to choose what wavelength to use in post-processing
All systems can be fully serviced and supported onsite
- No need to worry about shipping systems for service or support, all units can be fully serviced onsite to ensure minimum downtime
How it works
Following separation of the sample according to its hydrodynamic volume the sample will pass through 1 or more detectors where its presence is recorded. OMNISEC REVEAL is a fully integrated multi-detection module which can contain up to 4 different detectors.
The RI and UV detectors give information about the concentration of the components in the sample. The light scattering detectors respond to the intensity of light scattered by the sample which is related to molecular weight and also allows Rg of large molecules to be calculated. The viscometer measures the changing solution viscosity to calculate the intrinsic viscosity of the sample which is a measure of structure.
Coupling the efficient sample separation of OMNISEC RESOLVE with the fully optimized, multi-detector, information-rich analysis delivered by OMNISEC REVEAL, Malvern Panalytical has produced a market-leading solution for researchers and formulators developing novel natural and synthetic polymers, pharmaceutical and biopharmaceutical products and foods.
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Refractive Index
- Measure the concentration of any solute with excellent sensitivity
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UV/Vis PDA
- Measure absorbance at any wavelength from 190-900 nm
- Assess changes in absorbance spectra
- Characterize copolymers
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Light Scattering
- Measure absolute molecular weight in a wider range of applications
- Measure as little as 100 ng of material
- Measure molecular weights down to 200 Da
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Viscometer
- Better intrinsic viscosity measurements thanks to automated balancing
- Reduced service cost and time thanks to user-exchangeable capillaries
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Specification
System
Dimensions (W, D, H) |
420, 640, 600 mm |
Weight |
40 kg |
Detector temperature range |
20 - 65 °C |
Software |
OMNISEC software v10 (or later) |
Data acquisition rate |
100 Hz |
Patents |
US20140060162A1 & EP2619543B1 "Modular capillary bridge viscometer"
US20140144214A1 & EP2619544A1 "Automatically balanced capillary bridge viscometer"
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Detector 1
Detector |
Light scattering |
Measurement principle |
RALS 90° angle, LALS 7° angle |
Light source |
50 mW, 640 nm laser |
Cell volume |
18 µL |
Dynamic range |
2500 mV |
Baseline noise |
<0.1 mV |
Baseline drift |
<0.2 mV/hr |
Detector 2
Detector |
Differential refractive index |
Measurement principle |
Deflection |
Cell volume |
12 µL |
Dynamic range |
±2.5 x 10-4 RIU |
Baseline noise |
<10-7 RIU |
Baseline drift |
<3 x 10-7 RIU/hr |
Detector 3
Detector |
Viscometer |
Measurement principle |
4-capillary Wheatstone bridge with self-balancing mechanism and user-exchangeable capillaries |
Detector volume |
17 µL/capillary |
Differential pressure dynamic range |
±2500 Pa |
Differential pressure baseline noise |
0.3 Pa |
Inlet pressure dynamic range |
100 kPa |
Inlet pressure baseline noise |
0.01 kPa |
Baseline drift |
<0.2 kPa/hr |
Detector 4
Detector |
Diode-array-based UV/Vis spectrometer |
Wavelength |
190 - 900 nm |
Number of wavelengths |
1024 |
Wavelength accuracy |
<1 nm |
Wavelength resolution |
0.6 nm |
Cell volume |
7.5 µL |
Path length |
10 mm |
Baseline noise |
2 x 10-5 AU |
Baseline drift |
5 x 10-4 AU/hr |
Measurement type 1
Measurement type |
Absolute molecular weight |
Measurement range |
200 - >107 g/mol |
Minimum quantifiable mass |
Using HPLC-SEC:
100 ng of 100 kDa molecular weight polystyrene in THF
100 ng of BSA in PBS
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Measurement principle |
Light scattering (LALS or RALS) |
Measurement type 2
Measurement type |
Intrinsic viscosity |
Minimum quantifiable mass |
1 µg of 100 kDa molecular weight polystyrene in THF |
Measurement principle |
4-capillary Wheatstone bridge |
Measurement type 3
Measurement type |
Concentration |
Minimum quantifiable mass |
Using HPLC-SEC:
100 ng of 100 kDa molecular weight polystyrene in THF
100 ng of BSA in PBS |
Measurement principle |
Differential refractive index detection |
Operating environment
Temperature |
15 °C - 30 °C |
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