RNA interference(RNAi),specification,price,image-Bio-Equip in China

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RNAi (RNA interference) refers to the introduction of homologous double stranded RNA (dsRNA) to specifically target a gene's product, resulting in null or hypomorphic phenotypes. The use of antisense RNA to interfere with a gene's activity in C. elegans was first utilised by Su Guo and Ken Kemphues to studypar-1 ; however, it was reported that control sense RNA also produced a par-1 mutant phenotype (Cell 81: 611-20, 1995). Subsequently, it was discovered by Fire et al. '98 that it is the presence of dsRNA, formed from the annealing of sense and antisense strands present in the in vitro RNA preps, that is responsible for producing the interfering activity. Introduction of dsRNA into an adult worm results in the loss of the targeted endogenous mRNA from both the adult and its progeny. This phenomenon has been effectively harnessed to study an ever increasing number of maternal and zygotic genes in C. elegans.

The most interesting aspects of RNAi are the following:

dsRNA, rather than single-stranded antisense RNA, is the interfering agent
it is highly specific
it is remarkably potent (only a few dsRNA molecules per cell are required for effective interference)
the interfering activity (and presumably the dsRNA) can cause interference in cells and tissues far removed from the site of introduction