Real time PCR is a kinetic approach, where you look at the reaction in the early stages while it is still linear. There are many real time machines available. This is the one we use (the BioRad Icycler IQ real time PCR instrument). The lid slides back and then we put samples in a 96-well plate format inside, so one can look at a lot of samples simultaneously. The machine contains a sensitive camera which monitors the fluorescence in each well of the 96-well plate at frequent intervals during the PCR reaction. In our case, as DNA is synthesized, more SYBR green will bind and the fluorescence will increase.
BIOLINK will perform this experiment in the way as follows:
Design the specific primer and the fluorescence-labeled probe based on the gene sequence
Extract DNA/ RNA
Real Time PCR and analyze
Provide thorough results with both primer information and data analyzed by the software.
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