50% Transparent PC Membrane Cell Insert, Tissue Culture Treated
| Cat.No. |
Type
（Well) |
Pore Size
(um) |
Membrane Density |
Insert Membrane Growth Area（cm²） |
Insert Diameter
(mm) |
Well Volume
(ml) |
Insert Volume
(ml) |
packing specification |
| 723001 |
6 |
0.4 |
1 x 108 well/cm² |
4.67 |
24 |
2.6 |
1.5 |
6 inserts/6well plate 60 inserts/case |
| 724001 |
12 |
0.4 |
1 x 108 well/cm² |
1.12 |
12 |
1.5 |
0.5 |
12 inserts/12well plate 120 inserts/case |
| 725001 |
24 |
0.4 |
1 x 108well/cm² |
0.33 |
6.5 |
0.6 |
0.1 |
12 inserts/24well plate 120 inserts/case |
| 723101 |
6 |
3 |
2 x 106 well/cm² |
4.67 |
24 |
2.6 |
1.5 |
6 inserts/6well plate 60 inserts/case |
| 724101 |
12 |
3 |
2 x 106well/cm² |
1.12 |
12 |
1.5 |
0.5 |
12 inserts/12well plate 120 inserts/case |
| 725101 |
24 |
3 |
2 x 106well/cm² |
0.33 |
6.5 |
0.6 |
0.1 |
12 inserts/24well plate 120 inserts/case |
| 724201 |
12 |
5 |
4 x 105well/cm² |
1.12 |
12 |
1.5 |
0.5 |
12 inserts/12well plate 120 inserts/case |
| 725201 |
24 |
5 |
4 x 105well/cm² |
0.33 |
6.5 |
0.6 |
0.1 |
12 inserts/24well plate 120 inserts/case |
| 723301 |
6 |
8 |
1 x 105 well/cm² |
4.67 |
24 |
2.6 |
1.5 |
6 inserts/6well plate 60 inserts/case |
| 724301 |
12 |
8 |
1 x105well/cm² |
1.12 |
12 |
1.5 |
0.5 |
12 inserts/12well plate 120 inserts/case |
| 725301 |
24 |
8 |
1 x105well/cm² |
0.33 |
6.5 |
0.6 |
0.1 |
12 inserts/24well plate 120 inserts/case |
50% Transparent PC Membrane Cell Insert, Non-treated
| Cat.No. |
Type
（Well) |
Pore Size
(um) |
Membrane Density |
Insert Membrane Growth Area（cm²） |
Insert Diameter
(mm) |
Well Volume
(ml) |
Insert Volume
(ml) |
packing specification |
| 723011 |
6 |
0.4um |
1 x 108 well/cm² |
4.67 |
24 |
2.6 |
1.5 |
6 inserts/6well plate 60 inserts/case |
| 724011 |
12 |
0.4um |
1 x108 well/cm² |
1.12 |
12 |
1.5 |
0.5 |
12 inserts/12well plate 120 inserts/case |
| 725011 |
24 |
0.4um |
1 x 108 well/cm² |
0.33 |
6.5 |
0.6 |
0.1 |
12 inserts/24well plate 120 inserts/case |
| 723111 |
6 |
3um |
2 x106 well/cm² |
4.67 |
24 |
2.6 |
1.5 |
6 inserts/6well plate 60 inserts/case |
| 724111 |
12 |
3um |
2 x 106 well/cm² |
1.12 |
12 |
1.5 |
0.5 |
12 inserts/12well plate 120 inserts/case |
| 725111 |
24 |
3um |
2 x 106well/cm² |
0.33 |
6.5 |
0.6 |
0.1 |
12 inserts/24well plate 120 inserts/case |
| 724211 |
12 |
5um |
4 x 105well/cm² |
1.12 |
12 |
1.5 |
0.5 |
12 inserts/12well plate 120 inserts/case |
| 725211 |
24 |
5um |
4 x 105well/cm² |
0.33 |
6.5 |
0.6 |
0.1 |
12 inserts/24well plate 120 inserts/case |
| 723311 |
6 |
8um |
1 x 105 well/cm² |
4.67 |
24 |
2.6 |
1.5 |
6 inserts/6well plate 60 inserts/case |
| 724311 |
12 |
8um |
1 x 105 well/cm² |
1.12 |
12 |
1.5 |
0.5 |
12 inserts/12well plate 120 inserts/case |
| 725311 |
24 |
8um |
1 x 105 well/cm² |
0.33 |
6.5 |
0.6 |
0.1 |
12 inserts/24well plate 120 inserts/case |
50% Transparent PC Membrane Cell Insert, Tissue Culture Treated
| Cat.No. |
Name |
Pore Size
(um) |
Membrane Density |
Insert Membrane Growth Area（cm²） |
Insert Diameter
(mm) |
Well Volume
(ml) |
Insert Volume
(ml) |
packing specification |
| 726001 |
100mm Dish |
3um |
2 x 106 well/cm² |
44 |
75mm |
13ml |
9ml |
10 inserts/case |
Transparent PET Membrane Cell Insert, Tissue Culture Treated
| Cat.No. |
Type
（Well) |
Pore Size
(um) |
Membrane Density |
Insert Membrane Growth Area（cm²） |
Insert Diameter
(mm) |
Well Volume
(ml) |
Insert Volume
(ml) |
packing specification |
| 723121 |
6 |
0.4 |
1 x 108 well/cm² |
4.67 |
24 |
2.6 |
1.5 |
6 inserts/6well plate 60 inserts/case |
| 724121 |
12 |
0.4 |
1 x 108 well/cm² |
1.12 |
12 |
1.5 |
0.5 |
12 inserts/12well plate 120 inserts/case |
| 725121 |
24 |
0.4 |
1 x 108 well/cm² |
0.33 |
6.5 |
0.6 |
0.1 |
12 inserts/24well plate 120 inserts/case |
| 723021 |
6 |
3 |
2 x 106 well/cm² |
4.67 |
24 |
2.6 |
1.5 |
6 inserts/6well plate 60 inserts/case |
| 724021 |
12 |
3 |
2 x 106well/cm² |
1.12 |
12 |
1.5 |
0.5 |
12 inserts/12well plate 120 inserts/case |
| 725021 |
24 |
3 |
2 x 106 well/cm² |
0.33 |
6.5 |
0.6 |
0.1 |
12 inserts/24well plate 120 inserts/case |
Transparent PET Membrane Cell Insert, Non-treated
| Cat.No. |
Type
（Well) |
Pore Size
(um) |
Membrane Density |
Insert Membrane Growth Area（cm²） |
Insert Diameter
(mm) |
Well Volume
(ml) |
Insert Volume
(ml) |
packing specification |
| 723131 |
6 |
0.4 |
1 x 108 well/cm² |
4.67 |
24 |
2.6 |
1.5 |
6 inserts/6well plate 60 inserts/case |
| 724131 |
12 |
0.4 |
1 x 108 well/cm² |
1.12 |
12 |
1.5 |
0.5 |
12 inserts/12well plate 120 inserts/case |
| 725131 |
24 |
0.4 |
1 x 108 well/cm² |
0.33 |
6.5 |
0.6 |
0.1 |
12 inserts/24well plate 120 inserts/case |
| 723031 |
6 |
3 |
2 x 106 well/cm² |
4.67 |
24 |
2.6 |
1.5 |
6 inserts/6well plate 60 inserts/case |
| 724031 |
12 |
3 |
2 x 106 well/cm² |
1.12 |
12 |
1.5 |
0.5 |
12 inserts/12well plate 120 inserts/case |
| 725031 |
24 |
3 |
2 x 106 well/cm² |
0.33 |
6.5 |
0.6 |
0.1 |
12 inserts/24well plate 120 inserts/case |
Multiple well plate or dish is made from PS
Permeable supports, also known as cell culture inserts, are an essential tool for the study of both anchorage-dependent and independent cell lines.
This product produce a cell culture environment that closely resembles an in vivo state. Allow polarized cells to carry out metabolic activities in a more natural manner because the cells feed both apically and basolaterally.
Application: co-culture, epithelial cell polarity, migration, invasion, transport and permeability studies.
·Innovative hanging design facilitates pipetting.
·Supplied in multiple well plates: 6-well, 12-well, 24well.
·PC membrane: low adsorption rate, reducing the loss of small molecular proteins and other compounds
·Transparent PET inserts permit sufficient optical transparency for visualization of cell outlines by phase contrast microscopy.
· Transparent PET membrane is easy to observe.
·The PC membrane is not easy to be torn or curled, and it is easy to operate when taking out the chamber.
·Compatible with most solvents used for fixing and dyeing.
Attention：
• Attachment of cells grown on a permeable support (membrane) is sensitive to the initial seeding density, so the initial use should be inoculated with different density guarantee optimal growth;
•During the cultivation process, the liquid should be taken through the gap between the upper layer and the hole. When adding liquid, be careful and slow to avoid the damage of the membrane;
•Incubation of the permeable support in the medium prior to culturing the cells can improve cell attachment and distribution.
Guidelines for us：
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight. • Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Check the volume of the culture medium regularly and add fresh medium as needed.
• The cell layer can be directly fixed and stained in the nest using basic cytological methods. Note Avoid using solvents that dissolve the PC film.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage.
Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
bio-equip.cn
A leading life science plastic consumables manufactory.
Wuxi NEST Biotechnology Co., Ltd. a leading life science plastic consumables manufactory, who is integrated with R&D production and sales, was established in 2009, located in Wuxi, Jiangsu, China. Our products have been exported to North America, Europe, Japan, Korea, India and other countries, enjoys an excellent reputation nationwide and abroad. Customers are almost all over the world.
2011, NEST passed the standard of quality management system of ISO 9001.
2014, NEST passed the standard of quality management system of ISO 11137.
2016, NEST passed the standard of quality management system of ISO 13485.
2020, NEST obtained the medical device production license.
Beside these certifications, we also gained CE and FDA standard.
NEST Scientific Inc. NJ, USA
With the development of the business, in order to send the products to customers in North America more quickly, establish deeper communication and cooperation relationship with oversea customers. NEST Scientific Inc. as a branch of Wuxi NEST Biotechnology Co., Ltd. established in New Jersey, USA in 2013. Composed by professional team with rich experience of training and sales skills, can provide professional training and communicate with customers in depth level, understand customers’ demands more quickly.
One of your best choice of medical and laboratory consumables.
NEST dedicated to research and develop innovation plastic consumables suitable for life sciences research and medical establishments. There are more 600 plastic consumables can be widely used for cell culture, molecular biology, immunoassays, liquid handling and storage. Such as cell culture plates, Erlenmeyer flasks, BioFactory and pipette tips, etc. More than 100 medical plastic consumables and reagents can be used in molecular diagnosis and vaccination, such as disposable samplers, transport media, swabs, nucleic acid extraction kits and disposable intranasal atomization device. In order to provide more comprehensive and convenient service, depend on the technique of our affiliated company – Wuxi Tech-star Technology Co., Ltd. We also provide lab instruments, such centrifuges, metal baths and Bio Bank, etc.
Quality guarantee
Experienced team & a complete plastic consumables production line.
We have a complete plastic consumables production line. Mold-making, injection molding and sterilization, all the steps are done efficiently in our own factory. Precision molds, high quality raw material and advanced equipments, producing and quality testing with strict and comprehensive rules. To ensure our products meet the highest quality and performance standards expected by the hospitals, research institutions and industrial, clinical laboratories that we are served.
Excellent ability of mold-making.
R&D team with more 30 years industry experiences, high quality mold-making equipments which imported from Germany, and professional mold analysis software, allow NEST could develop more precision molds.
Injection molding in Class 10,000 and Class 100,000 clean rooms, with strictly quality control.
10,764 ft2 Class 10,000 clean room and 32292 ft2 Class 100,000 clean room, all-electric high-speed injection molding machine imported from Japan, raw material which meet USP Class VI requirements. Production and quality control are performed strictly in accordance with corresponding SOP.
Sterilization by electronic beam irradiation.
The Rhodotron TT200 electron accelerator which imported from the IBA company, Belgium. A world leader in the E-Beam sterilization industry. Sterilization process has been certified by the ISO 11137 quality system. Compared with sterilization by cobalt 60 or ethylene oxide sterilization, sterilization by electronic beam irradiation, is more efficiently and safety. It costs less time and without any chemical residues.
Sales Manger:Frank
E-mail:frank@nest-wuxi.com
Skype:franknest
Whatsapp/Mobie:+86-15961801211
NEST Scientific USA
Tel : +1-732 381 0268
E-mail : info@nestscientificusa.com
Add : 1592 Hart St., Rahway, NJ07065, USA
中文
