The RNAscope® 2.5 High Definition (HD)—BROWN Assay, is based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. RNAscope® Assays do not require the RNA-free environment used for traditional ISH. The HD version is the most sensitive RNA ISH method and can be used for low expressing gene targets.

Sample Images For RNAscope 2.5 HD Assay - BROWN




RNAscope® 2.5 HD Reagent Kit—BROWN is the ideal starter kit for first time users. This very robust assay gives reliable results which are ideal for archiving due to the permanent staining. It is the standard in molecular pathology diagnostics, usable for a wide source of samples and the results are visible under any standard bright field microscope. The RNAscope® 2.5 HD - Brown Reagent Kits are designed to provide more intense Diaminobenzidine (DAB) staining when low copy target gene expression is anticipated (1–20 copies per cell). The RNAscope® 2.5 HD reagent kits are compatible with the same RNAscope® probes used previously with RNAscope® 2.5 assays.

The RNAscope® 2.5 HD—BROWN Manual Assay Workflow Requires:

RNAscope® Target Probes (Catalog or Made-to-Order channel 1 (C1) Probes)
RNAscope® Control Probes (Select species-specific positive control probes and negative control probes)
RNAscope® Control Slides
RNAscope® 2.5 HD Reagent Kit—BROWN (Pretreatment Kit, Detection kit, and Wash Buffer included)
Brightfield microscope
HybEZ™ Hybridization System

Bio-Techne Corporation (NASDAQ: TECH) is a leading developer and manufacturer of high quality purified proteins:notably cytokines and growth factors, antibodies, immunoassays, as well as biologically active small molecule compounds and ACD's in situ hybridization detection products,which are sold to biomedical researchers and clinical research laboratories; these operations constitute the core Biotechnology Division, headquartered in Minneapolis, Minnesota. The Protein Platforms Division manufactures innovative protein analysis tools under the ProteinSimple brand name that greatly automate western blotting and immunoassay practices. The Diagnostics Division manufactures FDA-regulated controls, calibrators, blood gas and clinical chemistry controls and other reagents for OEM customer and clinical customers. Bio-Techne products are integral components of scientific investigations into biological processes and the nature and progress of specific diseases. They aid in drug discovery efforts and provide the means for accurate clinical tests and diagnoses. With thousands of products in its portfolio, Bio-Techne generated approximately $563 million in net sales in fiscal 2017 and has approximately 1,800 employees worldwide. For more information on Bio-Techne and its brands, please visit www.bio-techne.com.