EliKine™ Rat VEGF ELISA Kit employs a two-site sandwich ELISA to quantitate VEGF in samples. An antibody specific for VEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

The EliKine™ VEGF Rat ELISA Kit contains below components:

Rat VEGF microplate

Rat VEGF standard

Rat VEGF detect antibody

EliKine™ Streptavidin-HRP

Standard diluent

Assay buffer

HRP substrate

Stop solution

Wash buffer

Plate covers.

The Rat VEGF ELISA Kit with the calibration range is: 15.6 pg/ml-1000 pg/ml, and sensitivity is: 8 pg/mL.

More Abbkine EliKine™ series of ELISA Kits at EliKine™.
 

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.