EliKine™ Human Leptin ELISA Kit employs a two-site sandwich ELISA to quantitate Leptin  in samples. An antibody specific for Leptin  has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Leptin  present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Leptin  is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Leptin  bound in the initial step. The color development is stopped and the intensity of the color is measured.

The EliKine™ Leptin Human ELISA Kit contains below components:

Human Leptin  microplate

Human Leptin  standard

Human Leptin  detect antibody

EliKine™ Streptavidin-HRP

Standard diluent

Assay buffer

HRP substrate

Stop solution

Wash buffer

Plate covers.

The Human Leptin ELISA Kit with the calibration range is: 31.25 pg/mL- 2000 pg/mL, and sensitivity is: 16 pg/mL.

More Abbkine EliKine™ series of ELISA Kits at EliKine™.
 

 

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.