C1-INH is a critically important protein that controls activation of multiple plasma mediator pathways. This protein, a member of the serine protease inhibitor (serpin) group, originally was described as an inhibitor of C1. It binds stoichiometrically to the active sites on both C1r and C1s to form a complex C1-INH-C1r-C1s-C1-INH and thus inhibits activated C1. In addition, C1-INH has been reported to remove the intact C1qrs complex from an activating surface and to inhibit autoactivation of C1. C1-INH is a known inhibitor of kinin generating (kallikrein), fibrinolytic (plasmin), and contact activation (intrinsic) pathway of the coagulation cascade. Recently, it has been shown to be an inhibitor of the mannan-binding lectin pathway of complement activation, inhibiting mannan-binding lectin-associated serine proteases (MASPs) in that pathway.

Rat Complement 1 inhibitor (C1INH) ELISA Kit employs a two-site sandwich ELISA to quantitate C1INH in samples. An antibody specific for C1INH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyC1INH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C1INH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C1INH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Complement 1 inhibitor (C1INH) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.