The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. Zona pellucida sperm-binding protein 2 is a structural component of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies.

Rat Zona pellucida sperm-binding protein 2 (ZP2) ELISA Kit employs a two-site sandwich ELISA to quantitate ZP2 in samples. An antibody specific for ZP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Zona pellucida sperm-binding protein 2 (ZP2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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