CSE is an enzyme which breaks down cystathionine into cysteine and alpha-ketobutyrate. Pyridoxal phosphate is a prosthetic group of this enzyme.The bioavailability of cysteine is rate-limiting for the hepatic synthesis of glutathione. Cysteine is provided through either the diet or the transsulfuration pathway, in which cystathionine derived from methionine is transformed into cysteine by cystathionine gamma-lyase.Mutant mice lacking CTH displayed pronounced hypertension and diminished endothelium-dependent vasorelaxation. Cystathionine was physiologically activated by calcium and calmodulin , which is a mechanism for H2S formation in response to vascular activation. Yang et al. (2008) concluded that their findings provided direct evidence that H2S is a physiologic vasodilator and regulator of blood pressure.

Rat Cystathionine gamma-lyase (CSE) ELISA Kit employs a two-site sandwich ELISA to quantitate CTH in samples. An antibody specific for CTH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCTH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Cystathionine gamma-lyase (CSE) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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