Alkaline phosphatase, intestinal also known as ALPI is a type of alkaline phosphatase.The deduced polypeptide showed 86.5% amino acid identity to placental ALP and 56.6% amino acid identity to liver/bone/kidney ALP. Thus, the immunologic cross-reactivity of ALPI and ALPP is perhaps explained.Calf Intestinal Alkaline Phosphatase (CIP) is an enzyme that catalyzes the 5' phosphate group's removal from DNA. This enzyme is frequently used in DNA sub-cloning, because DNA fragments which lack the 5' phosphoryl termini cannot self-ligate. Preventing self ligation is important both in improving the yield of properly ligated product, and reducing the background of improperly self-ligated contaminant.

Rat Intestinal-type alkaline phosphatase (ALPI) ELISA Kit employs a two-site sandwich ELISA to quantitate ALPI in samples. An antibody specific for ALPI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyALPI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALPI is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALPI bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Intestinal-type alkaline phosphatase (ALPI) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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