Apolipoprotein B100 is a protein that plays a role in metabolism. It is a form of low density lipoprotein (LDL). Apo-B levels correlate with the risk of coronary disease. The protein occurs in the plasma in 2 main isoforms, APOB48 and APOB100. The first is synthesized exclusively by the small intestine, the second by the liver. Both isoforms are coded by APOB and by a single mRNA transcript larger than 16 kb. . Apo-B100 is the major physiological ligand for the LDL receptor. Apo-B100 is a large monomeric protein, containing 4536 amino acids. Apo-B100 is synthesized in the liver and is required for the assembly of VLDL.It does not interchange between lipoprotein particles, as do the other apolipoproteins, and it is found in IDL and LDL after the removal of the Apo-A, E and C. Apo-B48 is present in chylomicrons and their remnants. It is essential for the intestinal absorption of dietary lipids.

Rat Apolipoprotein B100 (APO-B100) ELISA Kit employs a two-site sandwich ELISA to quantitate APOB in samples. An antibody specific for APOB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAPOB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APOB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APOB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Apolipoprotein B100 (APO-B100) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.