Glial Cell Line-derived Neurotrophic Factor (GDNF) is a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy.The protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease.

Rat Glial cell line-derived neurotrophic factor (GDNF) ELISA Kit employs a two-site sandwich ELISA to quantitate GDNF in samples. An antibody specific for GDNF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGDNF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GDNF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GDNF bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Glial cell line-derived neurotrophic factor (GDNF) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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