MAPRE3 is a member of the RP/EB family of genes. The protein localizes to the cytoplasmic microtubule network and binds APCL, a homolog of the adenomatous polyposis coli tumor suppressor gene.The predicted 282-amino acid protein is 54% identical to MAPRE1. Northern blot analysis revealed expression of a 2.2-kb transcript predominantly in brain and muscle. GST pull-down analysis determined that a homologous region in the C termini of APC and APCL binds to MAPRE3. Immunofluorescence and confocal microscopy demonstrated that MAPRE3 is localized in the microtubule network and colocalizes with APCL in the perinucleus and microtubule network.

Rat Microtubule-associated protein RP/EB family member 3 (MAPRE3) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPRE3 in samples. An antibody specific for MAPRE3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPRE3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPRE3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPRE3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Microtubule-associated protein RP/EB family member 3 (MAPRE3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.