Scramblases are members of the general family of transmembrane lipid transporters known as flippases. The inner-leaflet facing the inside of the cell contains negatively-charged amino-phospholipids and phosphatidylethanolamine. The outer-leaflet, facing the outside environment, contains phosphatidylcholine and sphingomyelin. Scramblase is an enzyme, present in the cell membrane, that can transport (scramble) the negatively-charged phospholipids from the inner-leaflet to the outer-leaflet, and vice versa.Wiedmer et al. (2000) determined that the PLSCR1 gene contains 9 exons and spans about 30 kb. The first exon is untranslated. By deletion analysis of PLSCR1 promoter-reporter constructs, they found that 97% of the promoter activity resides between -95 bp in the 5-prime flanking region to +60 bp of the first exon.

Rat Phospholipid scramblase 1 (PLSCR1) ELISA Kit employs a two-site sandwich ELISA to quantitate PLSCR1 in samples. An antibody specific for PLSCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLSCR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLSCR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLSCR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Phospholipid scramblase 1 (PLSCR1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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