Calcineurin, a calmodulin-regulated protein phosphatase, is found in the cells of all eukaryotes ranging from yeast to mammals. Wang et al. (1996) described this heterodimeric protein as having a 19-kD Ca(2+)-binding regulatory subunit, calcineurin B, and a 58- to 59-kD catalytic subunit, calcineurin A. One gene encodes calcineurin B in all tissues except testis, and it is highly conserved at the level of both protein and DNA sequences in eukaryotes. In contrast, there are 2 major isoforms, alpha and beta, of calcineurin A encoded by separate genes located on different human chromosomes. A third isoform, A-gamma, is unique to testis. Additional diversity of calcineurin A is created by alternative splicing of mRNAs. Calcineurin is especially abundant in brain where it constitutes 1% of total protein.

Rat Calcineurin subunit B type 1 (PPP3R1) ELISA Kit employs a two-site sandwich ELISA to quantitate PPP3R1 in samples. An antibody specific for PPP3R1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPP3R1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPP3R1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP3R1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Calcineurin subunit B type 1 (PPP3R1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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