The genes encoding the protein kinase C enzymes are widely distributed, e.g., PRKCA on chromosome 17, PRKCB1 on 16, and PRKCG on 19. Although the sequence homology among the PRKC family of genes is extensive, the pattern of expression varies among tissues. For example, the delta polypeptide appears to be the major isoform expressed in mouse hematopoietic cells. Mischak et al. (1991) isolated and characterized the mouse Prkcd gene.Aris et al. (1993) found that PKC-delta underwent calcium-independent autophosphorylation in the presence of phosphatidylserine and diacylglycerol. Diacylglycerol was an absolute requirement for PKC-delta activation. This and other cofactor and substrate requirements distinguished human PKC-delta from its mouse homolog.Organism species: Homo sapiens (Human)

Rat Protein kinase C delta type (PRKCD) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKCD in samples. An antibody specific for PRKCD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKCD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKCD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKCD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Protein kinase C delta type (PRKCD) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/rat-protein-kinase-c-delta-type-prkcd-elisa-kit-kte100429/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.