The hormonally responsive prolactin-inducible protein gene is expressed in benign and malignant breast tumor tissues and in some normal exocrine organs such as sweat, salivary, and lacrimal glands.The PIP gene is 7 kb long with 4 exons ranging from 106 bp to 223 bp.PIP is overexpressed in 80% of primary and metastatic breast cancers, is duplicated in the breast carcinoma cell line T47D. The 2 copies are organized as a large palindrome, lying 'in loco' on one chromosome 7. The FRA7I fragile site lies 2 Mb telomeric to the PIP gene and sets the distal end of the repeated sequence. Breakage at the FRA7I site was repaired by 1 cycle of the breakage--fusion-bridge (BFB) cycle mechanism, resulting in amplification of the PIP gene.

Rabbit Prolactin-inducible protein (PIP) ELISA Kit employs a two-site sandwich ELISA to quantitate PIP in samples. An antibody specific for PIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rabbit Prolactin-inducible protein (PIP) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.