Thromboxane A2 (TXA2) is a thromboxane. It is produced by activated platelets and has prothrombotic properties， it stimulates activation of new platelets as well as increases platelet aggregation. This is achieved by mediating expression of the glycoprotein complex GP IIb/IIIa in the cell membrane of platelets. Circulating fibrinogen binds these receptors on adjacent platelets, further strengthening the clot. TXA2 is generated from prostaglandin H2 by thromboxane-A synthase. Aspirin irreversibly inhibits platelet cyclooxygenase 1 preventing the formation of prostaglandin H2, and therefore thromboxane A2. TXA2 is very unstable in aqueous solution, since it is hydrolyzed within about 30 seconds to the biologically inactive thromboxane B2. Due to its very short half life, TXA2 primarily functions as an autocrine or paracrine mediator in the nearby tissues surrounding its site of production.

Rabbit Thromboxane A2 (TXA2) ELISA Kit employs a two-site sandwich ELISA to quantitate TXA2 in samples. An antibody specific for TXA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTXA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TXA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TXA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rabbit Thromboxane A2 (TXA2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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