POU5F2, Belongs to the POU transcription factor family. Class-5 subfamily. POU domain genes constitute a family of transcription factors that exhibit distinct temporal and spatial patterns of expression.In the brain, the highest expression levels were found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. mPOU is shown to bind to DNA sequences containing the octamer motif and other POU factor target sites. The distinct expression pattern and divergent DNA-binding characteristics indicate that mPOU may regulate a distinct set of genes.

Mouse POU domain, class 5, transcription factor 2 (POU5F2) ELISA Kit employs a two-site sandwich ELISA to quantitate POU5F2 in samples. An antibody specific for POU5F2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOU5F2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POU5F2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POU5F2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse POU domain, class 5, transcription factor 2 (POU5F2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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