Heat shock proteins or HSPs are being synthesized under different kind of stress conditions and act as molecular chaperones for protein molecules. Because these proteins were first found in cells that were exposed to high temperatures, they are called "heat shock proteins" and have been named according to their molecular weights. Hsp40 (heat shock protein 40 kD) is a mammalian heat shock protein. Its prokaryotic homolog is DnaJ.Hsp40 is a family of heat-shock proteins that contain a 70 amino-acid consensus sequence known as the J domain. The J domain of Hsp40 interacts with Hsp70 heat shock proteins. Hsp40 heat-shock proteins play a role in regulating the ATPase activity of Hsp70 heat-shock proteins.

Mouse Heat Shock Protein 40 (HSP40) ELISA Kit employs a two-site sandwich ELISA to quantitate HSP40 in samples. An antibody specific for HSP40 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHSP40 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSP40 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSP40 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Heat Shock Protein 40 (HSP40) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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