DNASE1L3 encodes a member of the DNase family. The protein hydrolyzes DNA, is not inhibited by actin, and mediates the breakdown of DNA during apoptosis. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.The predicted 306-amino acid protein was 45% identical to that of DNASE1. Northern blot analysis showed that DNASE1L3 was expressed primarily in spleen and liver cells. The predominant mRNA was 1.3 kb, although there were 2 other transcripts. The purified recombinant protein had DNase activity in vitro that, unlike that of DNASE1, was not inhibited by G-actin.

Mouse Deoxyribonuclease gamma (DNASE1L3) ELISA Kit employs a two-site sandwich ELISA to quantitate DNASE1L3 in samples. An antibody specific for DNASE1L3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNASE1L3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNASE1L3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNASE1L3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Deoxyribonuclease gamma (DNASE1L3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/mouse-deoxyribonuclease-gamma-dnase1l3-elisa-kit-kte71228/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.