DNA polymerase beta maintains genome integrity by participating in base excision repair. Over expression of POLB mRNA has been correlated with a number of cancer types whereas deficiencies in POLB results in hypersensitivity to alkylating agents, induced apoptosis and chromosomal breaking therefore it is essential that POLB expression is tightly regulated.POLB gene is upregulated by CREB1 transcription factor binding to the cAMP response element(CRE) present in the promoter of the POLB gene in response to exposure to alkylating agents. POLB gene expression is also regulated at the post transcriptional level as the 3’UTR of the POLB mRNA has been shown to contain three stem-loop structures that influence gene expression.
Mouse DNA polymerase beta (POLB) ELISA Kit employs a two-site sandwich ELISA to quantitate POLB in samples. An antibody specific for POLB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse DNA polymerase beta (POLB) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/mouse-dna-polymerase-beta-polb-elisa-kit-kte70715/
bio-equip.cn
Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.