ACADL is a protein belongs to the acyl-CoA dehydrogenase family, which is a family of mitochondrial flavoenzymes involved in fatty acid and branched chain amino-acid metabolism. This protein is one of the four enzymes that catalyze the initial step of mitochondrial beta-oxidation of straight-chain fatty acid. Defects in this gene are the cause of long-chain acyl-CoA dehydrogenase (LCAD) deficiency, leading to nonketotic hypoglycemia.Impaired ACADL activity results in non-ketotic hypoglycemia, hypotonia, muscle weakness and episodes of cardiorespiratory arrest associated with fasting. A number of straight-chain acyl-CoA dehydrogenases of different substrate specificities are present in mammalian tissues.

Mouse Long-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADL) ELISA Kit employs a two-site sandwich ELISA to quantitate ACADL in samples. An antibody specific for ACADL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACADL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACADL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACADL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Long-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADL) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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