The asialoglycoprotein receptor binds to desialylated (galactosyl-terminal) glycoproteins. It transports these glycoproteins via a series of membrane vesicles and tubules to an acidic-sorting organelle where the receptor and ligand dissociate.The ASGR1 gene encodes a glycoprotein that forms the asialoglycoprotein receptor, also known as the Ashwell receptor, which is specific for desialylated (galactosyl-terminal) glycoproteins and is expressed exclusively in hepatic parenchymal cells. Partially deglycosylated plasma glycoproteins are efficiently and specifically removed from the circulation via this receptor-ligand complex. Many functional studies describing the kinetics of ligand-binding, internalization, and recycling of such receptors have been performed on the human hepatoma cell line HepG2.

Human Asialoglyco protein receptor (ASGPR) ELISA Kit employs a two-site sandwich ELISA to quantitate ASGPR in samples. An antibody specific for ASGPR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyASGPR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ASGPR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ASGPR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Asialoglyco protein receptor (ASGPR) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.