Pleiotrophin was initially recognized as a neurite outgrowth-promoting factor present in rat brain around birth and as a mitogen toward fibroblasts isolated from bovine uterus tissue. Together with midkine these growth-factors constitute a family of secreted heparin-binding proteins now known as the Neurite Growth-promoting Factor (NEGF) family. During embryonic and early postnatal development, Pleiotrophin is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. Pleiotrophin is also expressed by several tumor cells and is thought to be involved in tumor angiogenesis. In the adult central nervous system.  Pleiotrophin is expressed in an activity-dependent manner in the hippocampus, where it can suppress long term potentiation induction.

Human Pleiotrophin (PTN) ELISA Kit employs a two-site sandwich ELISA to quantitate PTN in samples. An antibody specific for PTN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Pleiotrophin (PTN) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-pleiotrophin-ptn-elisa-kit-kte62598/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.