Betacellulin(BTC),has been identified in the conditioned medium of cell lines derived from mouse pancreatic beta cell tumors. Betacellulin is a 32-kilodalton glycoprotein that appears to be processed from a larger transmembrane precursor by proteolytic cleavage. The carboxyl-terminal domain of betacellulin has 50 percent sequence similarity with that of rat transforming growth factor alpha. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.Watanabe et al. purified soluble forms of human BTC to homogeneity from the conditioned medium of mouse A9 cells transfected with the human BTC precursor cDNA. Three types of soluble BTC, apparently derived from a single gene, were identified and their biologic activities assessed.

Human Beta cellulin (BTC) ELISA Kit employs a two-site sandwich ELISA to quantitate BTC in samples. An antibody specific for BTC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBTC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BTC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BTC bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Beta cellulin (BTC) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.