Product Description (Laser Speckle Blood Flow Imager)

An advanced imaging technology based on dynamic laser speckle analysis, specifically designed for animal experiments and clinical research. It enables real-time, non-invasive monitoring of microcirculatory blood flow dynamics in living tissues, providing a precise visualization tool for cardiovascular disease research, drug efficacy evaluation, neuroscience, wound healing, and other fields.

Laser Speckle Imaging Principle:
When a target is illuminated by a laser beam, the reflected light forms a random interference pattern (composed of bright and dark areas), known as a laser speckle pattern. If the target is stationary, the speckle pattern remains unchanged. However, movement of the target—such as red blood cell motion in tissues—causes fluctuations in the speckle pattern. The rate of speckle variation depends on the velocity of the moving target within the monitored area: faster movement results in more pronounced speckle changes. This variation is quantified as speckle contrast, which correlates with blood flow velocity. This principle forms the basis for assessing blood perfusion using laser speckle technology.

By analyzing microcirculatory blood flow parameters, the system evaluates vascular structure, microcirculatory function, and metabolic activity. It enables research into pathological mechanisms and microcirculatory changes during ischemia, hypoxia, stroke, inflammation, edema, hemorrhage, allergies, shock, tumors, burns, frostbite, and radiation damage. This technology holds significant value for scientific research, disease diagnosis, clinical analysis, and treatment strategies.

Product Features

1. High-Resolution Real-Time Imaging
122 fps high-speed imaging captures transient blood flow dynamics.
2688×1520 spatial resolution reveals microvascular networks and flow distribution details.

2. Non-Invasive Monitoring
Requires no contrast agents or invasive procedures, minimizing animal stress and ensuring data authenticity.
Supports long-term repeated observations for chronic disease model studies.

3. Multi-Scenario Adaptability
Compatible with mice, rats, rabbits, dogs, and other experimental animals, featuring adjustable mounts and adapters.

4. Intelligent Analysis Software
One-click generation of quantitative parameters (e.g., blood flow velocity, perfusion), with time-series analysis support.
3D blood flow reconstruction (optional) visualizes spatiotemporal flow distribution.

5. Portability & Scalability
Modular design allows lab-based or mobile cart deployment.
Integrates with EEG, temperature monitoring, and other modules for multimodal research.

Technical Highlights

1. Dynamic Speckle Algorithm Optimization: Patented motion artifact reduction ensures stable imaging in anesthetized or awake animals.
2. Ultra-Sensitive CMOS Sensor: Captures high signal-to-noise ratio images even in low-light conditions.
3. Thermal-Controlled Laser Safety: Complies with IEC 60825 standards (no protective goggles required).
4. Rapid Auto-Focus & Precision Electric Focus: Enhances efficiency across diverse scenarios.
5. Optical Lens Array: Filters environmental light interference for accurate perfusion data.

Applications

1. Basic Research
Ischemia-reperfusion injury, tumor angiogenesis, inflammatory response mechanisms.
Neurovascular coupling (e.g., cerebral blood flow monitoring in stroke models).

2. Drug Development
Dynamic efficacy evaluation of vasoactive drugs, anticoagulants, and anti-tumor agents.
Pharmacotoxicology studies (organ blood flow side-effect monitoring).

3. Veterinary Medicine
Peripheral vascular disease diagnosis (e.g., diabetic foot, burn recovery assessment).
Intraoperative real-time blood flow monitoring (e.g., flap transplantation, organ suturing).

Cases

1. A comparison of cerebral blood flow before and after subarachnoid hemorrhage (SAH) modeling in mice.

2. Mouse cerebral blood flow map [1]

(b) The cerebral blood flow (CBF) detected by the laser speckle blood flow imaging system before SAH, at 15 minutes and 24 hours after SAH surgery in Prox1-CreER T2 /Ctss f/f and Ctss f/f mice.

3. Mouse hindlimb ischemia

4. Rat mesenteric blood flow map

Technical Specifications

Parameter	Value
Resolution	2688*1520
Pixel Size	2.9*2.9μm
Max Frame Rate	122fps
Grayscale Bit Depth	16bit (12bit effective)
Color Bit Depth	24bit RGB
Imaging Laser	785nm, 0-100 mW
Output Formats	AVI video, TIFF images (24-bit RGB, 16-bit grayscale/pseudocolor), CSV data tables, HTML/PDF reports
Image Composition	Raw, laser (speckle), speckle comparison, pseudocolor, overlay
Shutter Mode	Rolling Shutter
Field of View	14mm×22mm~57mm×100mm
Working Distance	10-40 cm (adjustable)
Focus Mode	Auto-focus & electric fine-focus
Calibration	Includes calibration kit

References

[1] Chen J, Wang J, Zheng W, et al. Brain–cervical lymph node crosstalk contributes to brain injury induced by subarachnoid hemorrhage in mice[J]. Nature Communications, 2025, 16(1): 8551.

Service Support

1. Free Training: Comprehensive instruction on equipment operation and standardized protocols for animal experiments.
2. Global Warranty: 3-year warranty with lifetime technical support.
3. Custom Development: Tailored analysis modules or hardware expansions to meet specific research needs.

*Our company can provide 3Q verification, customized function services based on customers' special applications and needs, and related experimental services. For more details, please call us for a consultation.

TOW-Int, as a high-tech enterprise focusing on the research and development, production and sales of animal experimental equipment and cell research instruments, has always been committed to providing customers with cutting-edge, efficient and stable scientific research solutions.

TOW-Int has been widely recognized by customers at domestic and abroad, mainly due to its focus on the development and manufacturing of clinical animal experimental equipment, as well as many years of experience in the field of animal science experimental research. The company is committed to breaking the monopoly of foreign products, establishing a proud brand of Chinese manufacturing, and providing accurate solutions for global customers.