Sepax Zenix SEC Columns,specification,price,image-Bio-Equip in China

Product Detail
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Description

Made of 3 µm silica particles, Zenix SEC packing is a breakthrough technology for size exclusion chromatography.

Combination of 3 µm silica particle size and proprietary surface technology enables highest separation efficiency

and resolution for biological molecules and water soluble polymers. Available pore sizes of Zenix packings are

100, 150 and 300Å.

Sepax SEC Product Family Comparison Chart

Product Line	SRT®	Zenix™	SRT®-C	Zenix™-C
Particle size	5 µm	3 µm	5 µm	3 µm
Pore size (Å)	100, 150, 300, 500, 1000 & 2000	80, 100, 150, 300	100, 150, 300, 500, 1000 & 2000	80, 100, 150, 300
Resolution	High	Highest, Short column for faster separation	High	Highest, Short column for faster separation
Efficiency	High	Doubled from 5µm	High	Doubled from 5µm
Selectivity	Same for SRT® and Zenix™		Same for SRT®-C and Zenix™-C
Surface structure	Chemically bonded stand-up monolayer		Chemically bonded lay-down monolayer
Recommended Sample Types	Monoclonal antibodies, proteins, peptides, nucleic acids, oligonucleotides, virus, and water-soluble polymers		"Tough samples" such as hydrophobic proteins like insulin, membrane protein monoclonal antibodies derivatized with polymer branches, e.g. polypeptide, PEG.

Packing

Zenix S

EC Technical Specifications

Phase	Zenix SEC-80	Zenix SEC-100	Zenix SEC-150	Zenix SEC-300
Material	Neutral, hydrophilic film bonded silica
Particle size	3 µm
Pore size	~ 80 Å	~ 100 Å	~ 150 Å	~ 300 Å
Protein MW range (native)	100 - 50,000	100 - 100,000	500 - 150,000	5,000 - 1,250,000
pH stability	2 - 8.5 (pH 8.5-9.5 can be tolerated temporarily.)
Backpressure (psi for a 7.8×300 mm)	~ 1,500	~ 1,500	~ 1,500	~ 1,500
Maximum backpressure (psi)	~ 4,500	~ 4,500	~ 4,500	~ 3,500
Salt concentration range	20 mM - 2.0 M
Maximum temperature (°C)	~ 80
Mobile phase compatibility	Aqueous and organic

Characteristic

o Particle size of 3 µm

o Selection of pore size: 80, 100, 150 and 300 Å

o Highest separation efficiency and resolution

o High loading capacity

o High stability over low and high concentration salt

o Lot-to-lot reproducibility

o High protein recovery with intact biological activity

o Negligible non-specific interactions

o Ideal for separation and analysis of biological molecules: proteins, nucleic acids, oligonucleotides,

peptides and virus

o Ideal for separation and analysis of natural polymers, e.g. polysaccharides, synthetic polymers,

and nanomaterials, e.g. nanoparticles

Conditions
Column:	Zenix SEC-300 (3 µm, 7.8×300mm)
Mobile phase:	150 mM phosphate buffer, pH 7.0
Flow rate:	1.0 mL/min
Temperature:	Ambient
Detector:	UV 214 nm
Injection volume:	5 µL
Sample:	1. Thyroglobulin (1.0 mg/mL), 670 kD 2. BSA dimer, 132 kD 3. BSA (1.0 mg/mL), 66 kD 4. Ribonuclease A (1.0 mg/mL), 13.7 kD 5. Uracil (0.1 mg/mL), 120 D

Pore size vs. MW exclusion limit

Phases (3 µm)	Pore Size	Protein MW Exclusion Limit
Zenix SEC-80	80 Å	50,000
Zenix SEC-100	100 Å	100,000
Zenix SEC-150	150 Å	150,000
Zenix SEC-300	300 Å	1,250,000

Column Dimension Availability

Available Zenix SEC column dimensions are 0.75, 1.0, 2.1, 3.0, 4.6, 7.8, 10, 21.2 and 30 mm I.D., and 20, 30, 50, 100, 150, 250, 300 and 600 mm length. Sepax also offers custom-made columns. Both stainless steel and PEEK tubes are available.

High Separation Efficiency

The advantages of developing small particle size are higher efficiency and higher resolution. When particle size is decreased to 3 µm from 5 µm, the column efficiency is almost doubled. As shown in Table 1, the plate numbers of BSA dimmer, BSA, ribonuclease A increased from 2720 to 4600, 6590 to 13090, 11160 to 22000 when the particle size decreased from 5 µm to 3 µm. Fig. 2 and Fig. 3 further show that high efficiency has been achieved by 3 µm Zenix columns with various proteins. The efficiency of p-aminobenzoic acid reached to the plate number of 40,000 for 30 cm long Zenix column.

Figure 1. Separation of protein mixture A by Zenix SEC-300 and SRT SEC-300 columns.

Conditions
Column:	Zenix SEC-300 and SRT SEC-300
Mobile phase:	150 mM phosphate buffer, pH 7.0
Flow rate:	1.0 mL/min for 7.8×300mm, 0.35 mL/min for 4.6×300mm
Temperature:	Ambient
Detector:	UV 214 nm
Injection volume:	10 µL for 7.8×300mm, 3 µL for 4.6×300mm
Sample:	1. Thyroglobulin (1.0 mg/mL), 670 kD 2. BSA dimer, 132 kD 3. BSA (1.0 mg/mL), 66 kD 4. Ribonuclease A (1.0 mg/mL), 13.7 kD 5. Uracil (0.1 mg/mL), 120 D

Table 1. Efficiency of Zenix SEC-300 and SRT SEC-300 columns.

Peak	Protein	Zenix 300 (4.6×300)	Zenix 300 (7.8×300)	SRT 300 (7.8×300)
1	Thyroglobulin	2180	1730	1120
2	BSADimer	4390	4600	2720
3	BSA	10280	13090	6590
4	Ribonuclease A	16490	22000	11160
5	Uracil	33640	38500	27860

Figure 2. Separation of protein mixture B by Zenix SEC-150 and 300 columns with 7.8 mm ID.

Conditions
Column:	3 µm, 7.8×300mm
Mobile phase:	150 mM phosphate buffer, pH 7.0
Flow rate:	1.0 mL/min
Temperature:	Ambient
Detector:	UV 214 nm
Injection volume:	10 µL
Sample:	1. Thyroglobulin, 670 kD 2. γ-Globulin, 158 kD 3. Ovalbumin, 44 kD 4. Ribonuclease A, 13.7 kD 5. p-Aminobenzoic acid, 137 D

Table 2. Efficiency of 7.8×300 mm Zenix SEC-150 and 300 columns.

Peak	Protein	Zenix 150	Zenix 300
1	Thyroglobulin	12420	1295
2	γ-Globulin	2860	3650
3	Ovalbumin	6620	11760
4	Ribonuclease A	16450	21690
5	p-Aminobenzoic acid	40550	39400

Figure 3. Separation of protein mixture B by Zenix SEC-150 and 300 columns with 4.6 mm ID.

Conditions
Column:	3 µm, 4.6×300mm
Mobile phase:	150 mM phosphate buffer, pH 7.0
Flow rate:	0.35 mL/min
Temperature:	Ambient
Detector:	UV 214 nm
Injection volume:	5 µL
Sample:	1. Thyroglobulin, 670 kD 2. γ-Globulin, 158 kD 3. Ovalbumin, 44 kD 4. Ribonuclease A, 13.7 kD 5. p-Aminobenzoic acid, 137 D

Table 3. Efficiency of 4.6×300 mm Zenix SEC-150 and 300 columns.

Peak	Protein	Zenix 150	Zenix 300
1	Thyroglobulin	6020	2410
2	γ-Globulin	2560	3000
3	Ovalbumin	6030	10260
4	Ribonuclease A	13350	17020
5	p-Aminobenzoic acid	35500	33480

High Loading Capacity

Loading capacity is critical for size exclusion separation and purification. Figure 4 shows high loading capacity for BSA as one example (>500 µg for an analytical column).
Figure 4. BSA loading test on a Zenix SEC-150 column.

Conditions
Column:	Zenix SEC-150 (3 µm, 7.8×300 mm)
Mobile phase:	150 mM phosphate buffer, pH 7.0
Flow rate:	1.0 mL/min
Temperature:	Ambient
Detector:	UV 214 nm
Injection volume:	10 µL

Figure 5. BSA loading test on a Zenix SEC-300 column.

Conditions
Column:	Zenix SEC-300 (3 µm, 7.8×300 mm)
Mobile phase:	150 mM phosphate buffer, pH 7.0
Flow rate:	1.0 mL/min
Temperature:	Ambient
Detector:	UV 214 nm
Injection volume:	10 µL

High Stability

The proprietary stationary phases of Zenix SEC packings utilize densely bonded chemistry on the silica surface, which greatly hinders the diffusion of the molecules that would attack the bond of silica-stationary phase layer, thus enabling high stability over a wide range of pH from 2 to 8.5.

Conditions
Column:	Zenix 3 µm, 7.8×300 mm
Mobile phase:	150 mM phosphate buffer, pH 7.0
Flow rate:	1.0 mL/min
Temperature:	Ambient
Injection volume:	10 µL
Sample:	A) BSA B) Thyroglobulin (1.0 mg/mL) BSA (1.0 mg/mL) Ribonuclease A (1.0 mg/mL) Uracil (2.5 mg/mL)

Mobile Phase Compatibility

Zenix SEC phases are compatible with most aqueous buffers, such as ammonium acetate, phosphate, trizma and so on. Zenix SEC phases can tolerate high concentration of salts, such as 2.0 M. Furthermore, Zenix SEC columns are stable in both organic solvents, such as methanol, ethanol, THF, DMF, DMSO, and so on; as well as the mixture of water and organic solvents.

Lot-to-Lot Reproducibility

The controlled surface chemistry used to synthesize Zenix SEC phases makes the surface coating highly reproducible, leading to consistent column manufacturing. Separation variation from batch to batch is controlled to be within 5% for retention time.

Conditions
Column:	Zenix SEC-300 (3 µm, 7.8×150mm)
Mobile phase:	150 mM phosphate buffer, pH 7.0
Flow rate:	1.0 mL/min
Temperature:	Ambient
Detector:	UV 214 nm
Injection volume:	10 µL
Samples:	1) Thyroglobulin, 2) BSA dimer, 3) BSA, 4) Ribonuclease A, 5) Uracil

High Protein Recovery

Zenix SEC phases are hydrophilic and neutral. Proteins and other biological molecules have negligible nonspecific interactions with Zenix stationary phases. The protein adsorption to the silica surface is suppressed, leading to high recovery of intact proteins, maintaining the protein activity after separation. More than 95% recovery is achieved for BSA and lysozyme, the representatives for acidic and basic proteins, respectively.