Line-Gene. K is the newest system in Bioer''s range of PCR detection systems. It features more advanced implementations of Peltier and fiber optic technology and a new direct current power system.
The new instruments share the Peltier effect and fiber optic design features that provide the exceptional accuracy and stability of the Line-Gene instruments.The new units offer improved uniformity,stability and accuracy by utilizing a constant-current power supply and an advanced multi-point temperature control module.The Line-Gene.K has improved temperature control and more uniform heating throughout the sample block to improve the accuracy of PCR detection.
The Line-Gene.K series is available in a range of automated and manual models with the ability to handle one, two, three or four combinations of excitation and emission wavelengths.
15 items(just for you!)
●Volume adaptation—software automatically adjusts heat exchange to differences in sample volumes.
●New multipoint temperature monitoring allows more accurate and uniform temperature control throughout the heating block.
●Gradient function—creates a temperature gradient across the sample block. Maximum 24℃ difference between row 1 and row 24.
●Sample preservation—after completion of experiment, the system can automatically refrigerate samples until they are moved to storage.
●Use of direct current power supply improves thermal efficiency and reduces power consumption by 30%.
●Improved optics—greater efficiency in fiber optic system provides reduced signal to noise ratio.
●Automatic hot lid—provides constant pressure and software-managed temperature control.
●Configuration upgradable—manual versions of the instrument can be upgraded to automatic. Systems can be upgraded from 1 wavelength to 4 wavelengths.
●Numerous data ports—choose from serial(RS 232),USB and ethernet connections for PC data interface.
●Multiple tube styles—the sample block will accept several styles of 0.2ml tubes including 8-tube strips.
●Data protection—system preserves experimental data if there is a power interruption during operation.
●Automatic amplitude adjustment—the instrument detects the fluorescence strength in the samples and automatically adjusts to the correct system sensitivity.
●New software—a new generation of Line-Gene software provides even greater convenience and more powerful functions.
●Upload software instructions—define experiment instructions with easy-to-use PC- based software,then upload to instrument.
●Overheating protection—if the operating environment exceeds the temperature range for operation within specification, the instrument will shut down.At 35℃the instrument will sound a warning;it will turn off at 40℃.
SNPs Detection
Line-Gene system is a multicolor fluorescence detection system and can carry out the detection analysis of SNPs.
2nd Derivative Maximum:
This method automatically calculates the maximum second derivative value of every fluorescent curve and defines this value as the Ct value.
Fit Points:
The user sets baseline and a threshold values then draws a threshold line.Based on user instructions,the software will select a number of sample points on the fluorescence curve that are above baseline and in the logarithmic phase into a line.The intersection of this line with the threshold line is defined as the Ct value.
Model |
FQD-48A (A4)
(A4: means automatic
lid and 4 channels) |
FQD-48A (M2)
(M2: means manual
lid and 2 channels) |
Sample Capacity |
48×0.2ml |
48×0.2ml |
Reaction System |
10~100μl |
10~100μl |
Dynamics Range |
10~1010Copies |
10~1010Copies |
Max.Channel Number of
Fluorescence Detection |
4 Channels |
2 Channels |
Excitation Light Wavel
length (450-590mm) |
Standard Positioning：
F1: 470nm F2: 523nm
F3: 543n F4: 571nm |
F1: 470nm F2: 523nm |
Fluorescence Detecting
Wavelength (500-630nm) |
Standard Positioning：
F1: 525nm F2: 564nm
F3: 584n F4: 612nm |
F1: 525nm F2: 564nm |
Detecting Fluorescence |
FAM, SYBR Green I, HEX, VIC, TAMRA,
TEX RED, ROX |
FAM,SYBR GreenⅠ,HEX |
Temp. Range |
4℃~99.9℃ |
4℃~99.9℃ |
Heating Rate (Max) |
≥4.0℃/sec |
≥4.0℃/sec |
Cooling Rate |
≥4.0℃/sec |
≥4.0℃/sec |
Temp. Uniformity of Block |
≤±0.3℃ |
≤±0.3℃ |
Temp. Control Accuracy |
≤±0.1℃ |
≤±0.1℃ |
Gradient Temp. Range |
1℃~24℃ |
1℃~24℃ |
Hot-lid Temp. Range |
80℃~120℃ |
80℃~120℃ |
Operation System |
Windows 2000/XP |
Windows 2000/XP |
Power Supply |
AC220V 50Hz 500W |
AC220V 50Hz 500W |
Dimension (mm) |
520×450×320(L×W×H) |
520×450×320(L×W×H) |
Net Weight |
25Kg(Without computer) |
25Kg(Without computer) |
bio-equip.cn
As a Japanese wholly owned enterprise, BIOER TECHNOLOGY CO., LTD. is the manufacturing base of the Bio Flux Corporation whose headquarter lies in Tokyo, Japan. BIOER is committed to researching and manufacturing of hi-tech products for life science applications. Years of experience and technical know-how have been developed into three product lines： life science instrumentation, related reagents and disposables and general laboratory instrumentation,such as Real-time PCR systems, thermal cyclers, cooling & heating blocks,Nucleic Acid Purification Systems, HBV, HCV, HIV, GMO, nucleic acid purification kits and all kinds of constant temperature baths. These products are recognized in both the overseas and domestic markets and have won praise from customers all over the world, including: Hong Kong, Taiwan, Korea, Japan, India, Malaysia & USA.
BIOER moved to its new state of the art facility in September 2003 that occupies a floor space of 16,000 square meters . BIOER uses the latest computer technology throughout its production process and strictly follows international ISO 13485 standards aiming at creating a first-class brand and products with first-class after-sales services for its clients.
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