Description

This product uses a unique lysis buffer system to quickly release RNA from cultured cell samples for RT-qPCR reactions, eliminating the time-consuming and laborious RNA purification process, and only 7 minutes to obtain the required RNA template, with the 5× Direct RT Mix, 2× Direct qPCR Mix-Taqman provided by the kit can quickly and efficiently obtain real-time quantitative PCR results.

5× Direct RT Mix and 2× Direct qPCR Mix-Taqman have strong inhibitor tolerance and can perform efficient reversal and specific amplification using the lysate of the sample to be measured as a template. The reagent contains Foregene Reverse Transcriptase, Hot D-Taq DNA Polymerase, dNTPs, MgCl2, Reaction Buffer, PCR Optimizer and Stabilizer, which can be used with lysis buffer to quickly and easily detect samples, and has the characteristics of high sensitivity, specificity and stability.

Specification

QuickEasyTM Cell Direct RT-qPCR Kit-Taqman
Kit components 20μl qPCR Reaction System		DRT-01021	DRT-01022	Note
		200 T	1000 T
Part I	Buffer CL	4 ml	20 ml	Cell Lysis
	Foregene Protease Plus II	80 μl	400 μl
	Buffer ST	400 μl	1 ml × 2
Part II	DNA Eraser	80 μl	400 μl
	5×Direct RT Mix *	160 μl	800 μl	RT
	2× Direct qPCR Mix-Taqman*	1 ml × 2	1.7 ml × 6	qPCR
	20×ROX Reference Dye	40 μl	200 μl
	RNase-Free ddH2O	1.7 ml	10 ml
Instruction Manual		1 piece	1 piece

*:Cell Lysis, 5×Direct RT Mix, 2× Direct qPCR Mix-Taqman can be purchased separately, details are provided in Appendix 1 (PAGE 13).

Product features

- Simple, effective Cell Direct RT technology that takes as little as 7 minutes to get RNA samples.

- Sample requirements are small, and a minimum of 10 cultured cells can be used for experimentation.

- High throughput for rapid RNA acquisition of cultured cells such as 384, 96, 24, 12, and 6-well plates.

- DNA Eraser is able to quickly remove released genomes, greatly reducing the impact on subsequent experimental results.

- Optimized RT and qPCR systems enable two-step RT-PCR with more efficient reverse transcription, specificity, and stronger RT-qPCR reaction inhibitor tolerance.

Kit application

- Scope of application: Cultured cells.

- Sample lysis interpreted RNA: used only as a two-step RT-qPCR template.

- Kits can be used for the following purposes: gene regulatory expression analysis, allele testing, drug screening, etc.

Storage conditions

1. Shipping Conditions

The whole process of low temperature ice pack box transportation, to ensure that the kit is in the <4 °C state.

2. Storage conditions

Store part I at 4°C and Part II at -20°C.

The Foregene Protease Plus II should be stored at 4°C,not frozen at -20°C.

Reagent 2× Direct qPCR Mix-Taqman is stored at -20°C, or at 4°C for short-term use if used frequently (within 10 days).

Foregene Co., Ltd. is specialized in the molecular biology lab reagents, IVD raw materials, IVD Kits, and related products and services.Foregene is integrated in R&D, manufacturing and marketing together .

Established in 2011, Foregene has owned the world-class Direct PCR Platform,DNA-Only,RNA double spin column Isolation Platform. The main products include Direct PCR reagents, DNA/RNA isolation kits, PCR Master Mix, Genotyping kits, FISH Probe Kits, IVD Raw Materials, various IVD kits, and Lab Equipments. Foregene can provide the whole solution of IVD products based on our technology Platforms.

Becuse of the world-class leading technology, we are popular in domestic and overseas markets.Foregene’s high quality products are widely used in hospitals, universities, national research institutions, medical laboratories,CRO companies, bio-pharm companies, etc.

Looking forward to Cooperate with you.