Introduction
The fully automated digital PCR system provides an absolute method of absolute quantification of target DNA or RNA molecules with unparalleled accuracy, accuracy and sensitivity. The system is based on microfluidic technology digital PCR, which automatically completes the steps of nucleic acid extraction, sample generation, sample amplification, sample detection, data analysis, etc. After loading, it can be unattended, especially suitable for large-scale sample parallel detection.

Functional
The system sample generator and sample reader can realize seamless integration with the two modules of nucleic acid extraction and ddPCR amplification, and realize the ddPCR fully automatic workstation, which makes the digital PCR system more widely used. The automated system reduces manual error, makes the sample more stable, and makes the inspection more accurate, while saving time and cost.

1. The fluid control system for sample preparation is more user-friendly, with 8 channels of sample preparation;
2. Unique oil phase preparation patent, compared with commonly used oils, can avoid various factors that are not conducive to droplet stability, such as: bubbles;
3. Fully independent intellectual property rights of the chip production, the droplet formation size is uniform and stable, the chip realizes a single-layer tile structure, completely coated with droplet particles, avoiding cross-contamination, and is convenient for detection and analysis;
4. Multi-channel fluorescence imaging detection and analysis, complete multi-channel droplet scanning and photographing at one time, no signal degradation, and the data is more accurate;
5. The system is highly integrated, and all test data can be read by inputting blood samples;
6. The system realizes independent control of single modules to avoid crosstalk pollution between modules;
7. The waste recycling system is complete and completely independent of the main working environment to avoid mutual pollution;
8. The system is equipped with high-pressure negative pressure and ultraviolet sterilization. The instrument is simple and generous, and the maintenance is convenient.

Application

Genotyping
◎Genetic variation detection (SNP/SNV)
◎mitochondrial mutation detection
◎DNA methylation quantitative detection

Pathogen detection
◎HBV detection
◎HPV classification

NGS
◎Sequence library absolute quantification

Genetic analysis
◎low abundance miRNA, mRNA expression analysis

Environmental monitoring
◎Detection of different environmental samples such as soil and water

Pilot Gene Technology (Hangzhou) Co., Ltd.is a high-tech enterprise company specializing in the development, production, sales and service of life science instruments and reagents. The company uses PCR (Digital PCR, dPCR) technology to build a high-tech diagnostic technology platform for independent innovation, independently develops the production of digital PCR products, and is committed to the development of diagnostic reagents in the three major clinical fields of hereditary diseases, accurate diagnosis and treatment of tumor diseases and infectious diseases.

The company has a high level of multi-disciplinary research and development team and high quality senior sales team, and established the international top R & D center and GMP production base, won the "Eagle plan" enterprises and national high-tech enterprises to identify, and has nearly thirty independent intellectual property invention patents.

Through the implementation of modern management system and mutual benefit and win-win cooperation mechanism, the East China gene has continuously opened up the international research and development team, built a better production base and a rigorous quality system, striving for the continuous breakthrough and innovation of core technology, and striving to become the top upstream supplier in the field of domestic liquid inspection. Medical institutions, governments and people win a lot in order to make greater contributions to the cause of human health.