REAGEN^™Nitrofurantoin (AHD) ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of nitrofurantoin in fish, shrimp, meat (chicken ,beef, pork and hepar), eggs, honey.

The unique features of the kit are:

• Rapid (4 hours), high recovery (80% - 105%), and cost-effective extraction methods for various samples.
• High sensitivity (0.03 ng/g or ppb) and low detection limit (0.06 ng/g or ppb) for various samples.
• High reproducibility.
• A quick ELISA assay (less than 1 hours regardless of number of samples).

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The AHD-OVA has been coated in the plate wells. During the analysis, sample and the AHD antibody are added along with secondary antibody, tagged with a peroxidase enzyme. If the AHD residue is present in the sample, it will compete for the AHD antibody, thereby preventing the antibody from binding to the AHD-OVA attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the AHD residue concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN^™Nitrofurantoin (AHD) ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C*. The shelf life is 12 months when the kit is properly stored.

 

Kit Contents	Amount	Storage
AHD -Coated Plate	1 x 96well plate (8 wells x 12strips)	2-8°C
AHD Standards: Negative control (white cap tube) 0.03 ng/mL (yellow cap tube) 0.1 ng/mL (orange cap tube) 0.4 ng/mL (pink cap tube) 1 ng/mL (purple cap tube) 4 ng/mL (blue cap tube) 10 ng/mL(spiking,optional, red cap tube)	1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL	2-8°C       2-8°C
AHD Antibody (Antibody#1)	8 mL	2-8°C
HRP-Conjugated Antibody #2	6 mL
10X Sample Extraction Buffer	25 mL

REAGEN LLC is an indrustry-leading biomedical enterprise focused on designing and producing a broad range of ELISA kits, including clinical diagnostic kits, food and feed safety kits, and veterinary disease kits. we also manufacture microplate readers, plate washers, and related instruments and equipment.