Product Description

 

REAGEN™ Aflatoxin B1 ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Aflatoxin B1 in cereals, feed, milk, cookies, soy sauce, vinegar, oils, peanuts .The unique features of the kit are:

• High recovery (75-125%),rapid(10-40minutes), cost-effective extraction methods.
• High sensitivity (0.02 ng/g or ppb).
• High reproducibility.
• A quick ELISA assay (only 1 hour regardless of number of samples).

 

Procedure Overview

 

REAGEN™ The method is based on a competitive colorimetric ELISA assay. The Aflatoxin B1-BSA has been coated in the plate wells. During the analysis, sample and Aflatoxin B1 antibody (Antibody#1) and HRP-Conjugate (HRP-Conjugated Antibody #2) are added to the wells for incubation. If the Aflatoxin B1 residue is present in the sample, it will compete with the Aflatoxin B1 on the plate wells for the Aflatoxin B1 antibody, The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the Aflatoxin B1 residue concentration in the sample.

 

Kit Contents, Storage and Shelf Life

 

REAGEN™ Aflatoxin B1 ELISA Test Kit has the Aflatoxin B1acity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8℃ *. The shelf life is 12 months when the kit is properly stored.

Kit Contents	Amount	Storage
Aflatoxin B1-BSA-Coated Plate	1x 96-well Plate (8 wells x 12 strips)	2-8℃           2-8℃
Aflatoxin B1 Standards: Negative control (white cap tube) 0.02ng/mL (yellow cap tube) 0.06 ng/mL (orange cap tube) 0.2 ng/mL (pink cap tube) 0.6 ng/mL (purple cap tube) 1.5 ng/mL (blue cap tube)	1.0 mL 1.0.mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL
Antibody #1	6 m
HRP-Conjugated Antibody #2	6 ml
20X Wash Solution	30 mL
10 X Sample Dilution Buffer	25mL

REAGEN LLC is an indrustry-leading biomedical enterprise focused on designing and producing a broad range of ELISA kits, including clinical diagnostic kits, food and feed safety kits, and veterinary disease kits. we also manufacture microplate readers, plate washers, and related instruments and equipment.