REAGEN^™ Kanamycin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Kanamycin in meat/liver/kidney, cell lysate, urine, serum and milk.

The unique features of the kit are: 

• High recovery (>80%), rapid (10-40 minutes), and cost-effective extraction methods.
• High sensitivity (0.2 ng/g or ppb).
• High reproducibility.
• A quick ELISA assay (less than 1 hours regardless of number of samples).

Procedure Overview

 

The method is based on a competitive colorimetric ELISA assay. The Kanamycin-BSA has been coated in the plate wells. During the analysis, sample and Kanamycin antibody (Antibody #1) and HRP-Conjugate (HRP-Conjugated Antibody #2) are added to the wells for incubation. If the Kanamycin residue is present in the sample, it will compete with the  Kanamycin on the plate wells for the Kanamycin antibody, The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the Kanamycin residue concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN^™ Kanamycin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.

Kit Contents	Amount	Storage
Kanamycin-coated  Plate	1 x 96-well Plate	2-8°C
Kanamycin Standards  Negative control (white CAP tube) 0.2 ng/mL (yellow CAP tube) 0.6 ng/mL (orange CAP tube) 1.8 ng/mL (pink cap tube) 5.4 ng/mL(purple cap tube) 16.2 ng/mL (blue cap tube) 1000 ng/mL(spiking, optional, red cap tube)	1mL 1mL 1mL 1mL 1mL 1mL 1mL	2-8℃
Kanamycin Ab#1	6.0mL	2-8℃
HRP-Conjugated Ab#2	6.0mL
10×Sample diluent	15 mL
20× Wash Solution	28mL
Stop Buffer	12mL
TMB Substrate	12mL
10×Sample Ext. buffer (optional)	15mL
Sample Balance Buffer	2 mL

 

* If you are not planning to use the kit for over 1 month, store Kanamycin Standard Stock, Kanamycin Antibody #1and  HRP-Conjugated Antibody #2 at -20°C or in a freezer.

 

Sensitivity (Detection Limit)

 

Sample Type	Detection Limit (ng/g or ppb)
Fish/Shrimp/Meat/liver/kidney	8
Cell Lysate	2
Urine /Serum	5
Milk	2

 

Specificity (Cross-Reactivity)

 

Analytes	Cross-Reactivity (%)
Kanamycin	100
Streptomycin	< 0.1
Dihydrostreptomycin	< 0.1
Neomycin	< 0.1

 

Required Materials Not Provided With the Kit

 

• Microtiter plate reader (450 nm)
• Tissue Mixer (e.g. Omni Tissue Master Homogenizer)
• Vortex mixer (e.g. Genie Vortex mixer from VWR)   
• 10, 20, 100 and 1000 uL pipettes
• Multi-channel pipette: 50-300 uL (Optional)

REAGEN LLC is an indrustry-leading biomedical enterprise focused on designing and producing a broad range of ELISA kits, including clinical diagnostic kits, food and feed safety kits, and veterinary disease kits. we also manufacture microplate readers, plate washers, and related instruments and equipment.