Human Vitamin D (VD) ELISA Kit,specification,price,image-Bio-Equip in China

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For the quantitative determination of Human VD concentrations.
Reactivity: Human
Methode Type: Sandwich ELISA Detection
Quantity: 96 tests
Sample type： serum, plasma, Urine,tissue homogenates, cell culture supernates
Detection range : 30 nmol/L-1200nmol/L
Components:

Assay plate (12 × 8 coated Microwells)	1
Standard:2400 nmol/L	1×0.5ml
Standard Diluent	1×1.5ml
HRP-Conjugate Reagent	1×6ml
Sample Diluent	1×6ml
Chromogen Solution A	1×6ml
Chromogen Solution B	1×6ml
Stop Solution	1×6ml
Wash Solution	1×20ml×30 fold
User manual	1
Adhesive Strip	2

Product Principle:
The kit is for the quantitative level of VD in the sample, adopt purified Human VD antibody to coat microtiter plate, make
solid-phase antibody, then add VD to wells, Combine VD antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at
HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of VD in the samples is then determined by comparing the O.D. of the samples to
the standard curve.